Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade

ELISA analysis of peptide containing the histone modification of interest using GTX60352 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade.

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications and the unmodified sequence of histone H3 using GTX60352 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade.
Dilution : 1:20,000

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60352 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade.
Dilution : 1:1,000

ChIP analysis of sheared chromatin from 10⁴ HeLa cells using GTX60352 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade. Respectively 1 and 5 μg of the antibody and 5 μg of IgG (negative IP control) were used per ChIP experiment. QPCR was performed with primers for the GAPDH promoter and for the inactive gene MYOD. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K9me1 is preferably present at silent genes.