Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150]

ChIP analysis of HeLa cells using GTX60894 Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg

The GTX60894 reacts to Histone H3 monomethy-lated at Lysine 9 (K9me1). No cross reactivity with non- modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.

WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60894 Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150].
Dilution : 0.5μg/ml

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60894 Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150].
Red : Primary antibody
Green : Actin