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Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150]

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60894
Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150] used in Western Blot (WB). GTX60894
Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150] used in (). GTX60894
Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150] used in ChIP assay (ChIP assay). GTX60894

Cat. No. GTX60894

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM150

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, ChIP assay

Reactivity

 Human
Package
100 μg ($449)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2 μg/mL - 1 μg/mL
ICC/IF 0.5 μg/mL - 2 μg/mL
ELISA 0.2 μg/mL - 1 μg/mL
ChIP assay 2 μg/mL - 10 μg/mL
Not tested in other applications.

Product Note

This antibody reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys9).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888007

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60894

GTX60894 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60894 Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150].
Red : Primary antibody
Green : Actin

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150] used in Western Blot (WB). GTX60894

GTX60894 WB Image

WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60894 Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150].
Dilution : 0.5μg/ml

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150] used in (). GTX60894

GTX60894 Image

The GTX60894 reacts to Histone H3 monomethy-lated at Lysine 9 (K9me1). No cross reactivity with non- modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150] used in ChIP assay (ChIP assay). GTX60894

GTX60894 ChIP assay Image

ChIP analysis of HeLa cells using GTX60894 Histone H3K9me1 (Mono-methyl Lys9) antibody [RM150]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg

REFERENCE

Application Reference

Eid W et al. Biol Chem 2016; (Epub) Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A.
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REVIEW

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Package List Price ($)
$ 449