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Histone H3K9me1 (Mono-methyl Lys9) antibody

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12177
Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12177
Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in Western Blot (WB). GTX12177
Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12177

Cat. No. GTX12177

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Frog
Package
50 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
IHC-P Assay dependent
Not tested in other applications.

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic methylated peptide corresponding to amino acids 7-20 [Me-Lys9] of human histone H3. The sequence is identical in many species.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12177

GTX12177 IHC-P Image

IHC-P analysis of rat esophagus tissue using GTX12177 Histone H3K9me1 (monomethyl Lys9) antibody at 0.1 μg/mL.

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12177

GTX12177 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX12177 Histone H3K9me1 (monomethyl Lys9) antibody at 0.1 μg/mL.

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in Western Blot (WB). GTX12177

GTX12177 WB Image

WB analysis of HeLa cell lysate using GTX12177 Histone H3K9me1 (monomethyl Lys9) antibody.
Lane 1 : Positive control HeLa cells whole cell extract
Lane 2-3 : no inhibition with non-methylated Histone H3 peptide peptide (5 and 10 μg/mL)
Lane 4-5 : inhibition with the methylated (Me-Lys9) Histone H3 peptide (5 and 10 μg/mL)

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12177

GTX12177 IHC-P Image

IHC-P analysis of rat lung tissue using GTX12177 Histone H3K9me1 (monomethyl Lys9) antibody at 0.1 μg/mL.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399