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Histone H3K9me1 (Mono-methyl Lys9) antibody

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12177
Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12177
Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12177
Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in Western Blot (WB). GTX12177

Cat No. GTX12177

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Frog
Package
50 μl ($279)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 1% BSA

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic methylated peptide corresponding to amino acids 7-20 [Me-Lys9] of human histone H3. The sequence is identical in many species.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H3 histone family member 3A , H3.3A , H3F3

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12177

GTX12177 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX12177 Histone H3K9me1 (monomethyl Lys9) antibody at 0.1 μg/mL.

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12177

GTX12177 IHC-P Image

IHC-P analysis of rat esophagus tissue using GTX12177 Histone H3K9me1 (monomethyl Lys9) antibody at 0.1 μg/mL.

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12177

GTX12177 IHC-P Image

IHC-P analysis of rat lung tissue using GTX12177 Histone H3K9me1 (monomethyl Lys9) antibody at 0.1 μg/mL.

Anti-Histone H3K9me1 (Mono-methyl Lys9) antibody used in Western Blot (WB). GTX12177

GTX12177 WB Image

WB analysis of HeLa cell lysate using GTX12177 Histone H3K9me1 (monomethyl Lys9) antibody.
Lane 1 : Positive control HeLa cells whole cell extract
Lane 2-3 : no inhibition with non-methylated Histone H3 peptide peptide (5 and 10 μg/mL)
Lane 4-5 : inhibition with the methylated (Me-Lys9) Histone H3 peptide (5 and 10 μg/mL)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 279