APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1,000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
PROPERTIES
Form
Liquid
Buffer
0.01M PBS pH7.4, 1% BSA
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
synthetic methylated peptide corresponding to amino acids 7-20 [Me-Lys9] of human histone H3. The sequence is identical in many species.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
H3 histone family member 3A , H3.3A , H3F3
Cellular Localization
Nucleus
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX12177 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX12177 Histone H3K9me1 (monomethyl Lys9) antibody at 0.1 μg/mL.
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GTX12177 IHC-P Image
IHC-P analysis of rat esophagus tissue using GTX12177 Histone H3K9me1 (monomethyl Lys9) antibody at 0.1 μg/mL.
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GTX12177 IHC-P Image
IHC-P analysis of rat lung tissue using GTX12177 Histone H3K9me1 (monomethyl Lys9) antibody at 0.1 μg/mL.
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GTX12177 WB Image
WB analysis of HeLa cell lysate using GTX12177 Histone H3K9me1 (monomethyl Lys9) antibody.
Lane 1 : Positive control HeLa cells whole cell extract
Lane 2-3 : no inhibition with non-methylated Histone H3 peptide peptide (5 and 10 μg/mL)
Lane 4-5 : inhibition with the methylated (Me-Lys9) Histone H3 peptide (5 and 10 μg/mL)
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REFERENCE
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REVIEW
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