Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade

Cat. No. GTX60357

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, Dot, ELISA, ChIP assay
Reactivity	Human, Mouse, Arabidopsis thaliana, Rice, Xenopus, Tomato, Brassica napus
Package	50 μg ($429)

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Datasheet

View product citations for antibody GTX60357 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1,000
ICC/IF	1:500
Dot	1:20,000
ELISA	1:1,000
ChIP assay	1-5 μg

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region of histone H3 containing the dimethylated lysine 9 (H3K9me2), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60357 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60357 Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade.
Dilution : 1:1,000

GTX60357 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60357 Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers specific for promoter of the inactive HBB gene and the coding region of the inactive MYOD gene, used as positive controls, and for the promoters of the active genes c-fos and GAPDH, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

GTX60357 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60357 Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade.

GTX60357 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed NIH3T3 cells using GTX60357 Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

GTX60357 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and the unmodified H3K9 sequence using GTX60357 Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade.
Dilution : 1:20,000

REFERENCE

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REVIEW

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Package

List Price ($)

50 μg

$ 429

Qty

Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade

Cat. No. GTX60357

Host

Clonality

Isotype

Application

Reactivity