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Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade

Anti-Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade used in Western Blot (WB). GTX60357
Anti-Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade used in ELISA (ELISA). GTX60357
Anti-Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60357
Anti-Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade used in Dot blot (Dot). GTX60357
Anti-Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60357

Cat No. GTX60357

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, Dot, ELISA, ChIP assay
Reactivity Human, Mouse
Package
50 μg ($319)
APPLICATION

Application Note

ChIP (2 μg/ChIP), ELISA (1:1,000), Dot blotting (1:20,000), Western blotting (1:1,000), Immunofluorescence (1:500)
PROPERTIES

Form

Liquid

Buffer

PBS containing 0.05% azide and 0.05% ProClin 300.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Affinity purified polyclonal antibody

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

di-Methyl Lys9 Histone H3 Antibody, Histone H3 di-Methyl Lys9 Antibody, Histone H3 di-Methyl K9 Antibody, Histone H3 di Methyl Antibody, di Methyl K9 Histone H3 Antibody, di-Methyl K9 Histone H3 Antibody, di Methyl Histone H3 Antibody, histone cluster 1, H3a Antibody, H3/A Antibody, HIST1H3A Antibody, Histone H3 di-Methyl Antibody, H3FA Antibody, diMethyl Histone H3 Antibody, Histone H3 diMethyl Antibody, Methyl Histone H3 Antibody, diMethyl Lys9 Histone H3 Antibody, di Methyl Histone H3 Lys9 Antibody, di-Methyl Histone H3 Antibody, diMethyl K9 Histone H3 Antibody, Histone H3 Methyl Antibody, di Methyl Histone H3 K9 Antibody, di Methyl Lys9 Histone H3 Antibody

Research Area

DATA IMAGES
Anti-Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade used in Western Blot (WB). GTX60357

GTX60357 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using H3K9me2 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.

Anti-Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade used in ELISA (ELISA). GTX60357

GTX60357 ELISA Image

ELISA was performed using a serial dilution of Histone H3K9me2 (di-Methyl Lys9) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:103,000.

Anti-Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60357

GTX60357 ChIP assay Image

ChIP was performed with HeLa nuclear extract and either 2 μg of H3K9me2 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD or GAPDH promoter.

Anti-Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade used in Dot blot (Dot). GTX60357

GTX60357 Dot Image

Dot blot analysis of peptides containing modified histone H3 and H4 and the unmodified H3K9 sequence using H3K9me2 antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane for analysis.

Anti-Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60357

GTX60357 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using H3K9me2 antibody and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9me2 antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI.

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Package List Price ($)
$ 319