ChIP analysis of HeLa cells using GTX60890 Histone H3K9me2 (Di-methyl Lys9) antibody [RM151]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg

WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60890 Histone H3K9me2 (Di-methyl Lys9) antibody [RM151].
Dilution : 0.5μg/ml

The GTX60890 reacts to Histone H3 dimethylatedat Lysine 9 (K9me2). No cross reactivity with non-modified Lysine 9 (H3 1-19), monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in Histone H3.

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60890 Histone H3K9me2 (Di-methyl Lys9) antibody [RM151].
Red : Primary antibody
Green : Actin