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Histone H3K9me2 (di-methyl Lys9) antibody

Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54102
Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX54102
Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in Dot blot (Dot). GTX54102
Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX54102
Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX54102
Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in Western Blot (WB). GTX54102
Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in ChIP assay (ChIP assay). GTX54102

Cat. No. GTX54102

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, Dot, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Dot Assay dependent
ChIP assay 1:50 - 1:200
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887841

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54102

GTX54102 ICC/IF Image

ICC/IF analysis of 293T cells using GTX54102 Histone H3K9me2 (di-methyl Lys9) antibody.
Blue : DAPI

Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX54102

GTX54102 IHC-P Image

IHC-P analysis of rat liver tissue using GTX54102 Histone H3K9me2 (di-methyl Lys9) antibody.
Dilution : 1:50

Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in Dot blot (Dot). GTX54102

GTX54102 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX54102 Histone H3K9me2 (di-methyl Lys9) antibody.

Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX54102

GTX54102 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX54102 Histone H3K9me2 (di-methyl Lys9) antibody.
Dilution : 1:50

Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX54102

GTX54102 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX54102 Histone H3K9me2 (di-methyl Lys9) antibody.
Dilution : 1:50

Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in Western Blot (WB). GTX54102

GTX54102 WB Image

WB analysis of various sample lysates using GTX54102 Histone H3K9me2 (di-methyl Lys9) antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Histone H3K9me2 (di-methyl Lys9) antibody used in ChIP assay (ChIP assay). GTX54102

GTX54102 ChIP assay Image

ChIP analysis of 293 cell lysate using GTX54102 Histone H3K9me2 (di-methyl Lys9) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

REFERENCE

Application Reference

Fang Yan et al. BMC Cardiovasc Disord 2022; 22 (1) : 182 H3K9me2 regulation of BDNF expression via G9a partakes in the progression of heart failure.
Application : WB, ChIP assay
Reactivity : Rat
Li H et al. Chemosphere 2019; 244 : 125445 Aluminium-induced synaptic plasticity injury via the PHF8-H3K9me2-BDNF signalling pathway.
Application : WB
Zhang K et al. Mol Cancer 2018; 17 (1) : 153 Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.
Application : WB
Reactivity : Human
Eid W et al. Biol Chem 2016; (Epub) Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A.
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399