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Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in ELISA (ELISA). GTX60824
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in Western Blot (WB). GTX60824
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60824
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60824
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in Dot blot (Dot). GTX60824
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in Protein Array (Protein Array). GTX60824
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60824

Cat. No. GTX60824

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, Dot, ELISA, ChIP assay, Protein Array

Reactivity

 Human, Mouse, Yeast
Package
50 μg ($519)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:250
Dot 1:20,000/1:5,000
ELISA 1:1,000
ChIP assay 0.5-5 μg
Protein Array Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region of histone H3 containing the trimethylated lysine 9 (H3K9me3), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in ELISA (ELISA). GTX60824

GTX60824 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade.

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in Western Blot (WB). GTX60824

GTX60824 WB Image

WB analysis of whole cell (50 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3.1, H3.2 and H4 (lane 3, 4, 5, 6 and 7, respectively) using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade.
Dilution : 1:1,000

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60824

GTX60824 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as negative controls, and for ZNF510 and the Sat2 satellite repeat, used as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60824

GTX60824 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:250

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in Dot blot (Dot). GTX60824

GTX60824 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K9 using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade.
Dilution : 1:20,000

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in Protein Array (Protein Array). GTX60824

GTX60824 Protein Array Image

Protein Array analysis of an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade. This figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.
Dilution : 1:5,000

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60824

GTX60824 ChIP assay Image

ChIP analysis of sheared chromatin from 1x10⁶ HeLa cells using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2A shows the signal distribution along the long arm of chromosome 19 and a zoomin to an enriched region containing several ZNF repeat genes. The arrows indicate two satellite repeat regions which exhibit a stronger signal. Figure 2C and D show the enrichment at the KCNQ1 and H19 imprinted genes.
Antibody amount : 1μg

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Package List Price ($)
$ 519