ChIP analysis of sheared chromatin from 1x10⁶ HeLa cells using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2A shows the signal distribution along the long arm of chromosome 19 and a zoomin to an enriched region containing several ZNF repeat genes. The arrows indicate two satellite repeat regions which exhibit a stronger signal. Figure 2C and D show the enrichment at the KCNQ1 and H19 imprinted genes.
Antibody amount : 1μg

Protein Array analysis of an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade. This figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.
Dilution : 1:5,000

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K9 using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade.
Dilution : 1:20,000

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:250

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as negative controls, and for ZNF510 and the Sat2 satellite repeat, used as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

WB analysis of whole cell (50 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3.1, H3.2 and H4 (lane 3, 4, 5, 6 and 7, respectively) using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade.
Dilution : 1:1,000

ELISA analysis of peptide containing the histone modification of interest using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade.