Histone H3K9me3 (Tri-methyl Lys9) antibody [HL2149]

Cat. No. GTX638127

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2149
Isotype	IgG
Application	WB, ICC/IF, Dot
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet

View product citations for antibody GTX638127 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
Dot	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys9 of human Histone H3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX638127 Dot Image

Dot blot analysis of anti-Histone H3K9me3 (Tri-methyl Lys9) antibody [HL2149] with peptide samples. Peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3K9me3 (Tri-methyl Lys9) antibody [HL2149] (GTX638127) varied dilution.

GTX638127 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K9me3 (Tri-methyl Lys9) antibody [HL2149] (GTX638127) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX638127 ICC/IF Image

Histone H3K9me3 (Tri-methyl Lys9) antibody [HL2149] detects Histone H3K9me3 (Tri-methyl Lys9) protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K9me3 (Tri-methyl Lys9) stained by Histone H3K9me3 (Tri-methyl Lys9) antibody [HL2149] (GTX638127) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

Histone H3K9me3 (Tri-methyl Lys9) antibody [HL2149]

Cat. No. GTX638127

Host

Clonality

Clone Name

Isotype

Application

Reactivity