Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade

Cat. No. GTX60327

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, Dot, ELISA, ChIP assay
Reactivity	Human
Package	50 μl ($399)

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Datasheet

View product citations for antibody GTX60327 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500
ICC/IF	1:500
Dot	1:1000
ELISA	1:1,000 - 1:10,000
ChIP assay	5 μl

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Histone H3 containing trimethylated lysine 9 and the phosphorylated serine 10 (H3K9me3S10p), using a KLH-conjugated synthetic peptide.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60327 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications and unmodified sequences of histone H3 using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade.
Dilution : 1:1,000

GTX60327 WB Image

WB analysis of 15 μg of histone extracts of HeLa cells treated with colcemid (to block the cell cycle in metaphase) using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade.
Dilution : 1:500

GTX60327 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade.

GTX60327 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

GTX60327 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁴ HeLa cells treated with colcemid using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade. A titration of the antibody consisting of 1, 5, and 10 μl per ChIP experiment was analysed. Additionally, the same titration was analysed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes GAPDH and c-fos and for the heterochromatin marker Sat2. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

REFERENCE

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REVIEW

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Package

List Price ($)

50 μl

$ 399

Qty

Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade

Cat. No. GTX60327

Host

Clonality

Isotype

Application

Reactivity