ChIP analysis of sheared chromatin from 10⁴ HeLa cells treated with colcemid using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade. A titration of the antibody consisting of 1, 5, and 10 μl per ChIP experiment was analysed. Additionally, the same titration was analysed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes GAPDH and c-fos and for the heterochromatin marker Sat2. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

ELISA analysis of peptide containing the histone modification of interest using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade.

WB analysis of 15 μg of histone extracts of HeLa cells treated with colcemid (to block the cell cycle in metaphase) using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade.
Dilution : 1:500

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications and unmodified sequences of histone H3 using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade.
Dilution : 1:1,000