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Histone H3R17me1 (mono-methyl Arg17) antibody

Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in Dot blot (Dot). GTX54131
Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in IHC (Paraffin sections) (IHC-P). GTX54131
Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54131
Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in Western Blot (WB). GTX54131
Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54131
Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54131
Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in Western Blot (WB). GTX54131
Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in IHC (Paraffin sections) (IHC-P). GTX54131
Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in IHC (Paraffin sections) (IHC-P). GTX54131

Cat No. GTX54131

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, Dot

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Dot Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic mono-methylated peptide corresponding to residues surroundingArg17 of human histone H3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone cluster 1 H3 family member j , H3/j , H3FJ

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in Dot blot (Dot). GTX54131

GTX54131 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX54131 Histone H3R17me1 (mono-methyl Arg17) antibody.

Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in IHC (Paraffin sections) (IHC-P). GTX54131

GTX54131 IHC-P Image

IHC-P analysis of rat lung tissue using GTX54131 Histone H3R17me1 (mono-methyl Arg17) antibody.
Dilution : 1:100

Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54131

GTX54131 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54131 Histone H3R17me1 (mono-methyl Arg17) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in Western Blot (WB). GTX54131

GTX54131 WB Image

WB analysis of various sample lysates using GTX54131 Histone H3R17me1 (mono-methyl Arg17) antibody.
Loading : 25μg per lane

Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54131

GTX54131 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX54131 Histone H3R17me1 (mono-methyl Arg17) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54131

GTX54131 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54131 Histone H3R17me1 (mono-methyl Arg17) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in Western Blot (WB). GTX54131

GTX54131 WB Image

WB analysis of C6 cell lysate using GTX54131 Histone H3R17me1 (mono-methyl Arg17) antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in IHC (Paraffin sections) (IHC-P). GTX54131

GTX54131 IHC-P Image

IHC-P analysis of human breast tissue using GTX54131 Histone H3R17me1 (mono-methyl Arg17) antibody.
Dilution : 1:100

Anti-Histone H3R17me1 (mono-methyl Arg17) antibody used in IHC (Paraffin sections) (IHC-P). GTX54131

GTX54131 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX54131 Histone H3R17me1 (mono-methyl Arg17) antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf