Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and H4 and unmodified sequences from histone H3 using GTX60344 Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade.
Dilution : 1:20,000

ELISA analysis of peptide containing the histone modification of interest using GTX60344 Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade.

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60344 Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade.
Dilution : 1:250

ChIP analysis of sheared chromatin from 1.6x10⁶ U2OS cells using GTX60344 Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade. A titration of the antibody consisting of 2, 5, 10 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1A: QPCR performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene. Figure 1B: QPCR performed with primers for the promoter of the active ALDOA gene and for the coding region of the inactive MYOD gene..