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Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade

Anti-Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60344
Anti-Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade used in Western Blot (WB). GTX60344
Anti-Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade used in ELISA (ELISA). GTX60344
Anti-Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade used in Dot blot (Dot). GTX60344

Cat. No. GTX60344

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, Dot, ELISA, ChIP assay

Reactivity

 Human
Package
50 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250
Dot 1:20,000
ELISA 1:1,000 – 1:3,000
ChIP assay 10 - 15 μl
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Histone H3 containing the asymmetrically dimethylated arginine 17 (H3R17me2(asym)), using a KLH-conjugated synthetic peptide.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60344

GTX60344 ChIP assay Image

ChIP analysis of sheared chromatin from 1.6x10⁶ U2OS cells using GTX60344 Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade. A titration of the antibody consisting of 2, 5, 10 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1A: QPCR performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene. Figure 1B: QPCR performed with primers for the promoter of the active ALDOA gene and for the coding region of the inactive MYOD gene..

Anti-Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade used in Western Blot (WB). GTX60344

GTX60344 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60344 Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade.
Dilution : 1:250

Anti-Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade used in ELISA (ELISA). GTX60344

GTX60344 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60344 Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade.

Anti-Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade used in Dot blot (Dot). GTX60344

GTX60344 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and H4 and unmodified sequences from histone H3 using GTX60344 Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade.
Dilution : 1:20,000

REFERENCE

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REVIEW

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Package List Price ($)
$ 399