GeneTex
United States (US)

Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody

GTX124113_41171_WB_18121410_783.jpg
GTX124113_41171_dot_blot_18121410_509.jpg
GTX124113_41171_IHC_M_2_18121410_785.jpg
GTX124113_41171_IHC_M_3_18121410_529.jpg
GTX124113_41171_IHC_18121410_935.jpg
GTX124113_41171_IHC_M_18121410_905.jpg

Cat No. GTX124113

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, Dot
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Dot Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3R17me2 (dimethyl Arg17). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES
GTX124113_41171_WB_18121410_783.jpg

GTX124113 WB Image

Histone3 (di-methyl Arg17) antibody detects HIST1H3A protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
15 % SDS-PAGE
Histone3 (di-methyl Arg17) antibody (GTX124113) dilution: 1:1000

GTX124113_41171_dot_blot_18121410_509.jpg

GTX124113 Dot Image

Dotblot analysis of anti-Histone3 (di-methyl Arg17) antibody with peptide samples.
Peptide samples (0.1 µg) were spotted onto positively charged nylon membrane and blotted with Histone3 (di-methyl Arg17) antibody (GTX124113) at different dilution as indicated.
A: Peptide samples of Histone3 (di-methyl Arg17)
B: Peptide samples of Histone H3.1

GTX124113_41171_IHC_M_2_18121410_785.jpg

GTX124113 IHC-P Image

Histone H3R17me2 (dimethyl Arg17) antibody detects Histone H3R17me2 (dimethyl Arg17) protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H3R17me2 (dimethyl Arg17) antibody (GTX124113) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124113_41171_IHC_M_3_18121410_529.jpg

GTX124113 IHC-P Image

Histone H3R17me2 (dimethyl Arg17) antibody detects Histone H3R17me2 (dimethyl Arg17) protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Histone H3R17me2 (dimethyl Arg17) antibody (GTX124113) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124113_41171_IHC_18121410_935.jpg

GTX124113 IHC-P Image

Histone3 (di-methyl Arg17) antibody detects HIST1H3A protein at nucleus on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
Histone3 (di-methyl Arg17) antibody (GTX124113) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124113_41171_IHC_M_18121410_905.jpg

GTX124113 IHC-P Image

Histone H3R17me2 (dimethyl Arg17) antibody detects Histone H3R17me2 (dimethyl Arg17) protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
Histone H3R17me2 (dimethyl Arg17) antibody (GTX124113) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169