Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade

Cat. No. GTX60825

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, Dot, ELISA, ChIP assay
Reactivity	Human, Mouse
Package	50 μg ($429)

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Datasheet

View product citations for antibody GTX60825 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1,000
ICC/IF	1:500
Dot	1:20,000
ELISA	1:1,000
ChIP assay	1-5 μg

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region of histone H3 containing the asymmetrically dimethylated R17 and the acetylated lysine 18 (H3R17me2(asym)K18ac), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60825 ChIP assay Image

ChIP analysis of HeLa cells using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete human X-chromosome and a zoomin to a 500 kb region (figure 2A and B), and in two regions on chromosome 14 and 3 surrounding the c-fos and EIF4A2 positive control genes (figure 2C and D, respectively).
Antibody amount : 1μg

GTX60825 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed NIH3T3 cells using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

GTX60825 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade.

GTX60825 WB Image

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade.
Dilution : 1:1,000

GTX60825 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3R17K18 using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade.
Dilution : 1:20,000

GTX60825 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

REFERENCE

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REVIEW

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Package

List Price ($)

50 μg

$ 429

Qty

Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade

Cat. No. GTX60825

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Research Area

DATA IMAGES

GTX60825 ChIP assay Image

GTX60825 ICC/IF Image

GTX60825 ELISA Image

GTX60825 WB Image

GTX60825 Dot Image

GTX60825 ChIP assay Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.