GeneTex
United States (US)

Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody

GTX124114_41171_WB_18121410_843.jpg
GTX124114_41171_dot_blot_18121410_756.jpg

Cat No. GTX124114

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, Dot
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Dot Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3R2me2 (dimethyl Arg2). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

histone cluster 1 antibody, methyl Histone H3 antibody, H3a antibody, histone H3/d antibody, histone H3/i antibody, histone H3/h antibody, histone H3/j antibody, H3R2me2 antibody, Di methyl Histone H3 antibody, histone H3/c antibody, member A antibody, HIST1H3A antibody, HIST1H3C antibody, histone H3.1 antibody, HIST1H3F antibody, histone H3/a antibody, H3/A antibody, histone H3/f antibody, H3 histone family antibody, HIST1H3I antibody, histone 1 antibody, histone H3/k antibody, HIST1H3J antibody, HIST1H3D antibody, HIST1H3B antibody, histone H3/b antibody, HIST1H3E antibody, HIST1H3G antibody, histone H3/l antibody, Dimethyl Histone H3 antibody, HIST1H3H antibody, H3FA antibody

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Research Area

DATA IMAGES
GTX124114_41171_WB_18121410_843.jpg

GTX124114 WB Image

Histone3 (di-methyl Arg2) antibody detects HIST1H3A protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
15 % SDS-PAGE
Histone3 (di-methyl Arg2) antibody (GTX124114) dilution: 1:1000

GTX124114_41171_dot_blot_18121410_756.jpg

GTX124114 Dot Image

Dotblot analysis of anti-Histone3 (di-methyl Arg2) antibody with peptide samples.
Peptide samples (0.1 µg) were spotted onto positively charged nylon membrane and blotted with Histone3 (di-methyl Arg2) antibody (GTX124114) at different dilution as indicated.
A: Peptide samples of Histone3 (di-methyl Arg2)
B: Peptide samples of Histone H3.1

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169