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Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody

Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Western Blot (WB). GTX124114
Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Dot blot (Dot). GTX124114

Cat. No. GTX124114

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, Dot

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Dot Assay dependent
Not tested in other applications.

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding asymmetric di-methyl Arg2 of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2752252

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Western Blot (WB). GTX124114

GTX124114 WB Image

Histone3 (di-methyl Arg2) antibody detects HIST1H3A protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
15 % SDS-PAGE
Histone3 (di-methyl Arg2) antibody (GTX124114) dilution: 1:1000

Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Dot blot (Dot). GTX124114

GTX124114 Dot Image

Dotblot analysis of anti-Histone3 (di-methyl Arg2) antibody with peptide samples.
Peptide samples (0.1 μg) were spotted onto positively charged nylon membrane and blotted with Histone3 (di-methyl Arg2) antibody (GTX124114) at different dilution as indicated.
A: Peptide samples of Histone3 (di-methyl Arg2)
B: Peptide samples of Histone H3.1

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169