GeneTex
United States (US)

Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody

GTX54134_20180530_WB_18121410_860.jpg
GTX54134_20180530_Dot_18121410_516.jpg
GTX54134_20180530_ICCIF_18121410_239.jpg

Cat No. GTX54134

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, Dot
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:200
ICC/IF 1:50 - 1:200
Dot Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H3T Antibody , H3.4 Antibody , HIST3H3 Antibody , H3FT Antibody , H3/G Antibody

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES
GTX54134_20180530_WB_18121410_860.jpg

GTX54134 WB Image

WB analysis of various samples using GTX54134 Histone H3R2me2 (Asymmetric dimethyl Arg2) antibody.
Loading : 25μg per lane

GTX54134_20180530_Dot_18121410_516.jpg

GTX54134 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX54134 Histone H3R2me2 (Asymmetric dimethyl Arg2) antibody.

GTX54134_20180530_ICCIF_18121410_239.jpg

GTX54134 ICC/IF Image

ICC/IF analysis of 293T cell using GTX54134 Histone H3R2me2 (Asymmetric dimethyl Arg2) antibody.
Blue : DAPI

Package List Price ($)
$ 319