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Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody

Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in IHC (Paraffin sections) (IHC-P). GTX54134
Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54134
Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in IHC (Paraffin sections) (IHC-P). GTX54134
Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Western Blot (WB). GTX54134
Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in IHC (Paraffin sections) (IHC-P). GTX54134
Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Dot blot (Dot). GTX54134
Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54134
Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54134

Cat. No. GTX54134

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, Dot

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Dot Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in IHC (Paraffin sections) (IHC-P). GTX54134

GTX54134 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX54134 Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody.
Dilution : 1:100

Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54134

GTX54134 ICC/IF Image

ICC/IF analysis of 293T cells using GTX54134 Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody.
Blue : DAPI

Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in IHC (Paraffin sections) (IHC-P). GTX54134

GTX54134 IHC-P Image

IHC-P analysis of rat brain tissue using GTX54134 Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody.
Dilution : 1:100

Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Western Blot (WB). GTX54134

GTX54134 WB Image

WB analysis of various sample lysates using GTX54134 Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:500
Loading : 25μg per lane

Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in IHC (Paraffin sections) (IHC-P). GTX54134

GTX54134 IHC-P Image

IHC-P analysis of human colon tissue using GTX54134 Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody.
Dilution : 1:100

Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Dot blot (Dot). GTX54134

GTX54134 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX54134 Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody.

Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54134

GTX54134 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX54134 Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54134

GTX54134 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54134 Histone H3R2me2 (Asymmetric Di-methyl Arg2) antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

Application Reference

P Yu et al. Research Square 2024; PRMT6-mediated Transcriptional Activation of YTHDF2 Promotes Glioblastoma Migration, Invasion, and EMT via the Wnt–β-Catenin Pathway
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399