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Histone H3R8me2 (Symmetric Di-methyl Arg8) antibody

Anti-Histone H3R8me2 (Symmetric Di-methyl Arg8) antibody used in Western Blot (WB). GTX33906
Anti-Histone H3R8me2 (Symmetric Di-methyl Arg8) antibody used in Dot blot (Dot). GTX33906
Anti-Histone H3R8me2 (Symmetric Di-methyl Arg8) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33906

Cat No. GTX33906

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, Dot
Reactivity Human
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Dot Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

histone cluster 3 H3 , H3.4 , H3/g , H3FT , H3t

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES
Anti-Histone H3R8me2 (Symmetric Di-methyl Arg8) antibody used in Western Blot (WB). GTX33906

GTX33906 WB Image

WB analysis of various samples using GTX33906 Histone H3R8me2 (Symmetric dimethyl Arg8) antibody.
Loading : 25μg per lane

Anti-Histone H3R8me2 (Symmetric Di-methyl Arg8) antibody used in Dot blot (Dot). GTX33906

GTX33906 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX33906 Histone H3R8me2 (Symmetric dimethyl Arg8) antibody.

Anti-Histone H3R8me2 (Symmetric Di-methyl Arg8) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33906

GTX33906 ICC/IF Image

ICC/IF analysis of 293T cell using GTX33906 Histone H3R8me2 (Symmetric dimethyl Arg8) antibody.
Blue : DAPI

REFERENCE
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REVIEW
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Package List Price ($)
$ 319