Histone H3S10ph (phospho Ser10) antibody - ChIP grade

Cat. No. GTX60827

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IP, Dot, ELISA, ChIP assay
Reactivity	Human
Package	50 μl ($399)

See all Histone H3 products

Datasheet

View product citations for antibody GTX60827 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500
ICC/IF	1:200
IP	5 μl
Dot	1:20,000
ELISA	1:1,000 - 1:5,000
ChIP assay	1-10 μl

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Histone H3 containing the phosphorylated serine 10 (H3S10p), using a KLH-conjugated synthetic peptide.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60827 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade.

GTX60827 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁴ HeLa cells treated with colcemid using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade. A titration of the antibody consisting of 1, 5, and 10 μl per ChIP experiment was analysed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes c-fos and RPL30. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

GTX60827 WB Image

WB analysis of 15 μg of histone extracts of HeLa cells treated with TSA (figure 4A) or with colcemid (figure 4B) using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade.
Dilution : 1:500

GTX60827 IP Image

IP analysis of HeLa cells were treated with colcemid (to block the cell cycle in metaphase) using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade.
IP reaction : 5 μl antibody / 1x10⁴ cells
Lane 1 : Primary antibody
Lane 2 : Input
Lane 3 : No antibody control
Dilution : 1:500

GTX60827 ICC/IF Image

ICC/IF analysis of paraformaldehyde fixed HeLa asynchronous cells using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade. Phosphorylation of H3 on serine 10 occurs on condensed chromosomes during mitosis. This explains the dense staining of one of the cells (indicated with an arrow).
Green : Primary antibody
Blue : DAPI
Dilution : 1:200

GTX60827 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and H4 and with peptides containing unmodified sequences from histone H3 using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade.
Dilution : 1:20,000

REFERENCE

There are currently no references for Histone H3S10ph (phospho Ser10) antibody - ChIP grade (GTX60827). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for Histone H3S10ph (phospho Ser10) antibody - ChIP grade (GTX60827). Be the first to share your experience with this product.

Submit a Review

Package

List Price ($)

50 μl

$ 399

Qty

Histone H3S10ph (phospho Ser10) antibody - ChIP grade

Cat. No. GTX60827

Host

Clonality

Isotype

Application

Reactivity