Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and H4 and with peptides containing unmodified sequences from histone H3 using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade.
Dilution : 1:20,000

ICC/IF analysis of paraformaldehyde fixed HeLa asynchronous cells using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade. Phosphorylation of H3 on serine 10 occurs on condensed chromosomes during mitosis. This explains the dense staining of one of the cells (indicated with an arrow).
Green : Primary antibody
Blue : DAPI
Dilution : 1:200

IP analysis of HeLa cells were treated with colcemid (to block the cell cycle in metaphase) using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade.
IP reaction : 5 μl antibody / 1x10⁴ cells
Lane 1 : Primary antibody
Lane 2 : Input
Lane 3 : No antibody control
Dilution : 1:500

WB analysis of 15 μg of histone extracts of HeLa cells treated with TSA (figure 4A) or with colcemid (figure 4B) using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade.
Dilution : 1:500

ChIP analysis of sheared chromatin from 10⁴ HeLa cells treated with colcemid using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade. A titration of the antibody consisting of 1, 5, and 10 μl per ChIP experiment was analysed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes c-fos and RPL30. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

ELISA analysis of peptide containing the histone modification of interest using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade.