Histone H3S10ph (phospho Ser10) antibody [GT1195]

Cat. No. GTX00957

Host	Rabbit
Clonality	Monoclonal
Clone Name	GT1195
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all Histone H3 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
ICC/IF	1:50 - 1:200
IHC-P	1:50 - 1:200

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.3), 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Phospho-Histone H3 (S10).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Histone H3 phospho Ser10

Cellular Localization

Nucleus,Chromosome

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Database

Gene ID: 8350 H3C1
UniProt: P68431 H3C1

Research Area

DATA IMAGES

GTX00957 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody [GT1195] (GTX00957) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX00957 WB Image

WB analysis of NIH-3T3 cell extracts with/without nocodazole (50 ng/ml, 37ºC, 20 hrs) treatment or CIP (20μl/400μl, 37ºC, 1 hr) treatment using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Dilution : 1:1000
Loading : 25μg

GTX00957 WB Image

WB analysis of C6 cell extracts with/without nocodazole (50 ng/ml, 37ºC, 20 hrs) treatment or CIP (20μl/400μl, 37ºC, 1 hr) treatment using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Dilution : 1:1000
Loading : 25μg

GTX00957 WB Image

WB analysis of HeLa cell extracts with/without nocodazole (50 ng/ml, 37ºC, 20 hrs) treatment or CIP (20μl/400μl, 37ºC, 1 hr) treatment using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Dilution : 1:1000
Loading : 25μg

GTX00957 ICC/IF Image

ICC/IF analysis of PFA-fixed C6 cells using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Orange : Primary antibody
Blue : DAPI

GTX00957 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Orange : Primary antibody
Blue : DAPI

GTX00957 IHC-P Image

IHC-P analysis of human oophoroma tissue section using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

Histone H3S10ph (phospho Ser10) antibody [GT1195]

Cat. No. GTX00957

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX00957 WB Image

GTX00957 WB Image

GTX00957 WB Image

GTX00957 WB Image

GTX00957 ICC/IF Image

GTX00957 ICC/IF Image

GTX00957 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.