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Histone H3S10ph (phospho Ser10) antibody [HL1752]

Anti-Histone H3S10ph (phospho Ser10) antibody [HL1752] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637402
Anti-Histone H3S10ph (phospho Ser10) antibody [HL1752] used in Western Blot (WB). GTX637402
Anti-Histone H3S10ph (phospho Ser10) antibody [HL1752] used in IHC (Paraffin sections) (IHC-P). GTX637402
Anti-Histone H3S10ph (phospho Ser10) antibody [HL1752] used in IHC (Whole mount) (IHC-Wm). GTX637402

Cat. No. GTX637402

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1752

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser10 of Histone H3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone cluster 1 H3 family member a , H3/A , H3FA

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H3S10ph (phospho Ser10) antibody [HL1752] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637402

GTX637402 ICC/IF Image

Histone H3S10ph (phospho Ser10) antibody [HL1752] detects Histone H3S10ph (phospho Ser10) protein at chromosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde at 37ºC for 30 min.
Green: Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [HL1752] (GTX637402) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Histone H3S10ph (phospho Ser10) antibody [HL1752] used in Western Blot (WB). GTX637402

GTX637402 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody [HL1752] (GTX637402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3S10ph (phospho Ser10) antibody [HL1752] used in IHC (Paraffin sections) (IHC-P). GTX637402

GTX637402 IHC-P Image

Histone H3S10ph (phospho Ser10) antibody [HL1752] detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [HL1752] (GTX637402) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3S10ph (phospho Ser10) antibody [HL1752] used in IHC (Whole mount) (IHC-Wm). GTX637402

GTX637402 IHC-Wm Image

Histone H3S10ph (phospho Ser10) antibody [HL1752] detects Histone H3S10ph (phospho Ser10) protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [HL1752] (GTX637402) diluted at 1:100.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations