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Histone H3S10ph (phospho Ser10) antibody

Anti-Histone H3S10ph (phospho Ser10) antibody used in Western Blot (WB). GTX128116
Anti-Histone H3S10ph (phospho Ser10) antibody used in IHC (Whole mount) (IHC-Wm). GTX128116
Anti-Histone H3S10ph (phospho Ser10) antibody used in Western Blot (WB). GTX128116
Anti-Histone H3S10ph (phospho Ser10) antibody used in Immunoprecipitation (IP). GTX128116
Anti-Histone H3S10ph (phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX128116
Anti-Histone H3S10ph (phospho Ser10) antibody used in IHC (Whole mount) (IHC-Wm). GTX128116
Anti-Histone H3S10ph (phospho Ser10) antibody used in ChIP assay (ChIP assay). GTX128116
Anti-Histone H3S10ph (phospho Ser10) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128116
Anti-Histone H3S10ph (phospho Ser10) antibody used in Western Blot (WB). GTX128116
Anti-Histone H3S10ph (phospho Ser10) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128116
Anti-Histone H3S10ph (phospho Ser10) antibody used in Western Blot (WB). GTX128116

Cat No. GTX128116

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay
Reactivity Human, Mouse, Rat, Zebrafish
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm 1:100-1:500
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

HeLa , HeLa (100 ng/ml Nacodazole treatment for 24 hr) , U2OS(100ng/ml Nocodazole treatment for 24hr) , 4T1 (100ng/ml Nocodazole treatment for 24hr) , Rat2 (100 ng/ml Nocodazole treatment for 24 hr)

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H3S10ph (phospho Ser10). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES
Anti-Histone H3S10ph (phospho Ser10) antibody used in Western Blot (WB). GTX128116

GTX128116 WB Image

Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis.
A. 30 µg U2OS whole cell lysate/extract (untreated)
B. 30 µg U2OS whole cell lysate/extract (serum deprivation for 48hr)
C. 30 µg U2OS whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr
15% SDS-PAGE
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3S10ph (phospho Ser10) antibody used in IHC (Whole mount) (IHC-Wm). GTX128116

GTX128116 IHC-Wm Image

Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3S10ph (phospho Ser10) (GTX128116) antibody dilution: 1:100.

Anti-Histone H3S10ph (phospho Ser10) antibody used in Western Blot (WB). GTX128116

GTX128116 WB Image

Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis.
A. 30 µg 4T1 whole lysate/extract (untreated)
B. 30 µg 4T1 whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
15% SDS-PAGE
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3S10ph (phospho Ser10) antibody used in Immunoprecipitation (IP). GTX128116

GTX128116 IP Image

Histone H3S10ph (phospho Ser10) antibody immunoprecipitates HIST1H3B protein in IP experiments.
IP samples: 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr
A. 30 μg 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of HIST1H3B protein by 4 μg Histone H3S10ph (phospho Ser10) antibody (GTX128116)
15 % SDS-PAGE
The immunoprecipitated HIST1H3B protein was detected by Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-Histone H3S10ph (phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX128116

GTX128116 IHC-P Image

Histone H3 (phospho Ser10) antibody detects HIST1H3A protein at nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3S10ph (phospho Ser10) antibody used in IHC (Whole mount) (IHC-Wm). GTX128116

GTX128116 IHC-Wm Image

Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3S10ph (phospho Ser10) antibody (GTX128116) dilution: 1:100.

Anti-Histone H3S10ph (phospho Ser10) antibody used in ChIP assay (ChIP assay). GTX128116

GTX128116 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract treated with Nocodazole (100 ng/ml for 24 h) and 5 μg of either control rabbit IgG or anti-Histone H3S10ph (phospho Ser10) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.

Anti-Histone H3S10ph (phospho Ser10) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128116

GTX128116 ICC/IF Image

HIST1H3A antibody histone cluster 1, H3a protein by immunofluorescent analysis. Sample: RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: histone cluster 1, H3a protein stained by HIST1H3A antibody (GTX128116) diluted at 1:500. Red: Tubulin, alpha 1b protein stained by TUBA1B antibody (GTX11304) diluted at 1:500 . Blue: Hoechst 33342 staining.

Anti-Histone H3S10ph (phospho Ser10) antibody used in Western Blot (WB). GTX128116

GTX128116 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (5 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:2000.

Anti-Histone H3S10ph (phospho Ser10) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128116

GTX128116 ICC/IF Image

Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein at chromosome by confocal immunofluorescent analysis.
Sample: Mitotic HeLa cells were fixed in 2% paraformaldehyde for 30 min.
Green: Histone H3 (phospho Ser10) protein stained by Histone H3 (phospho Ser10) antibody (GTX128116) diluted at 1:500.
Red: alpha Tubulin antibody (GTX11304) diluted at 1:2500.
Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]

Anti-Histone H3S10ph (phospho Ser10) antibody used in Western Blot (WB). GTX128116

GTX128116 WB Image

Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein by western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract (untreated)
B. 30 µg Rat2 whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr)
15% SDS-PAGE
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169