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Histone H3S28ph (phospho Ser28) antibody

Anti-Histone H3S28ph (phospho Ser28) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128953
Anti-Histone H3S28ph (phospho Ser28) antibody used in IHC (Paraffin sections) (IHC-P). GTX128953
Anti-Histone H3S28ph (phospho Ser28) antibody used in IHC (Paraffin sections) (IHC-P). GTX128953
Anti-Histone H3S28ph (phospho Ser28) antibody used in Western Blot (WB). GTX128953
Anti-Histone H3S28ph (phospho Ser28) antibody used in Dot blot (Dot). GTX128953

Cat No. GTX128953

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, Dot

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Dot Assay dependent
Not tested in other applications.

Positive Control

HeLa (100ng/ml Nocodazole treatment for 24hr)

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H3S28ph (phospho Ser28). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES

Anti-Histone H3S28ph (phospho Ser28) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128953

GTX128953 ICC/IF Image

Histone H3 (phospho Ser28) antibody detects Histone H3 (phospho Ser28) protein at chromosome in assynchronous HeLa cells.
Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min.
Green: Histone H3 (phospho Ser28) protein stained by Histone H3 (phospho Ser28) antibody (GTX128953) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Histone H3S28ph (phospho Ser28) antibody used in IHC (Paraffin sections) (IHC-P). GTX128953

GTX128953 IHC-P Image

Histone H3 (phospho Ser28) antibody detects Histone H3 (phospho Ser28) protein at nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Histone H3 (phospho Ser28) antibody (GTX128953) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3S28ph (phospho Ser28) antibody used in IHC (Paraffin sections) (IHC-P). GTX128953

GTX128953 IHC-P Image

Histone H3 (phospho Ser28) antibody detects Histone H3 (phospho Ser28) protein at nucleus on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Histone H3 (phospho Ser28) antibody (GTX128953) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3S28ph (phospho Ser28) antibody used in Western Blot (WB). GTX128953

GTX128953 WB Image

Histone H3 (phospho S28) antibody detects Histone H3 (phospho S28) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr)
15 % SDS-PAGE
Histone H3 (phospho S28) antibody (GTX128953) dilution: 1:1000

Anti-Histone H3S28ph (phospho Ser28) antibody used in Dot blot (Dot). GTX128953

GTX128953 Dot Image

Dotblot analysis of anti-Histone H3 (phospho Ser28) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (phospho Ser28) antibody (GTX128953) 1:5000 dilution.
A: Peptide samples of H3S10 un
B: Peptide samples of H3S10ph
C: Peptide samples of H3S28 un
D: Peptide samples of H3S28ph

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169