Histone H3S28ph (phospho Ser28) antibody

Cat No. GTX32416

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($319)

See all Histone H3 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:2000
ICC/IF	1:20 - 1:100

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S28 of human HIST1H3B

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone cluster 1 H3 family member a , H3/A , H3FA

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Database

Gene ID: 8350 HIST1H3A
UniProt: P68431 HIST1H3A

Research Area

DATA IMAGES

GTX32416 WB Image

WB analysis of 293T cells (25ug/ml treated with EGF for 30 minutes after serum-starvation) using GTX32416 Histone H3S29ph (phospho Ser29) antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32416 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX32416 Histone H3S29ph (phospho Ser89) antibody.
Blue : DAPI

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REVIEW

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Package

List Price ($)

100 μl

$ 319

Qty

Histone H3S28ph (phospho Ser28) antibody

Cat No. GTX32416

Host

Clonality

Isotype

Application

Reactivity