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Histone H3T11ph (phospho Thr11) antibody

Orthogonal
Anti-Histone H3T11ph (phospho Thr11) antibody used in Western Blot (WB). GTX32417
Anti-Histone H3T11ph (phospho Thr11) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32417
Anti-Histone H3T11ph (phospho Thr11) antibody used in IHC (Paraffin sections) (IHC-P). GTX32417
Anti-Histone H3T11ph (phospho Thr11) antibody used in IHC (Paraffin sections) (IHC-P). GTX32417
Anti-Histone H3T11ph (phospho Thr11) antibody used in IHC (Paraffin sections) (IHC-P). GTX32417
Anti-Histone H3T11ph (phospho Thr11) antibody used in IHC (Paraffin sections) (IHC-P). GTX32417
Anti-Histone H3T11ph (phospho Thr11) antibody used in IHC (Paraffin sections) (IHC-P). GTX32417
Anti-Histone H3T11ph (phospho Thr11) antibody used in IHC (Paraffin sections) (IHC-P). GTX32417
Anti-Histone H3T11ph (phospho Thr11) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32417
Anti-Histone H3T11ph (phospho Thr11) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32417
Anti-Histone H3T11ph (phospho Thr11) antibody used in Western Blot (WB). GTX32417

Cat No. GTX32417

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($319)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding T11 of human HIST1H3B

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone cluster 1 H3 family member a , H3/A , H3FA

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H3T11ph (phospho Thr11) antibody used in Western Blot (WB). GTX32417

GTX32417 WB Image

Orthogonal

WB analysis of HeLa, NIH/3T3, and C6 cells treated by Nocodazole (50 ng/ml) at 37ºC for 20 hours using GTX32417 Histone H3T11ph (phospho Thr11) antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-Histone H3T11ph (phospho Thr11) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32417

GTX32417 ICC/IF Image

ICC/IF analysis of C6 cells using GTX32417 Histone H3T11ph (phospho Thr11) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3T11ph (phospho Thr11) antibody used in IHC (Paraffin sections) (IHC-P). GTX32417

GTX32417 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX32417 Histone H3T11ph (phospho Thr11) antibody.
Dilution : 1:100

Anti-Histone H3T11ph (phospho Thr11) antibody used in IHC (Paraffin sections) (IHC-P). GTX32417

GTX32417 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX32417 Histone H3T11ph (phospho Thr11) antibody.
Dilution : 1:100

Anti-Histone H3T11ph (phospho Thr11) antibody used in IHC (Paraffin sections) (IHC-P). GTX32417

GTX32417 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32417 Histone H3T11ph (phospho Thr11) antibody.
Dilution : 1:100

Anti-Histone H3T11ph (phospho Thr11) antibody used in IHC (Paraffin sections) (IHC-P). GTX32417

GTX32417 IHC-P Image

IHC-P analysis of rat liver tissue using GTX32417 Histone H3T11ph (phospho Thr11) antibody.
Dilution : 1:100

Anti-Histone H3T11ph (phospho Thr11) antibody used in IHC (Paraffin sections) (IHC-P). GTX32417

GTX32417 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX32417 Histone H3T11ph (phospho Thr11) antibody.
Dilution : 1:100

Anti-Histone H3T11ph (phospho Thr11) antibody used in IHC (Paraffin sections) (IHC-P). GTX32417

GTX32417 IHC-P Image

IHC-P analysis of rat testis tissue using GTX32417 Histone H3T11ph (phospho Thr11) antibody.
Dilution : 1:100

Anti-Histone H3T11ph (phospho Thr11) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32417

GTX32417 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX32417 Histone H3T11ph (phospho Thr11) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3T11ph (phospho Thr11) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32417

GTX32417 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32417 Histone H3T11ph (phospho Thr11) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3T11ph (phospho Thr11) antibody used in Western Blot (WB). GTX32417

GTX32417 WB Image

Orthogonal

WB analysis of HeLa, NIH/3T3, and C6 cells treated by nocodazole (50 ng/ml) at 37ºC for 20 hours using GTX32417 Histone H3T11ph (phospho Thr11) antibody.
Loading : 25ug per lane
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319