Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3T3ph (phospho Thr3) antibody [HL3381] (GTX641201) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.