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Histone H3T3ph (phospho Thr3) antibody [RM159]

Orthogonal
Anti-Histone H3T3ph (phospho Thr3) antibody [RM159] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60897
Anti-Histone H3T3ph (phospho Thr3) antibody [RM159] used in Western Blot (WB). GTX60897
Anti-Histone H3T3ph (phospho Thr3) antibody [RM159] used in IHC (Paraffin sections) (IHC-P). GTX60897
Anti-Histone H3T3ph (phospho Thr3) antibody [RM159] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60897

Cat. No. GTX60897

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM159

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($449)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1 μg/mL - 1 μg/mL
ICC/IF 0.5 μg/mL - 2 μg/mL
IHC-P 0.5 μg/mL - 2 μg/mL
ELISA 0.2 μg/mL - 1 μg/mL
Not tested in other applications.

Product Note

This antibody reacts to Histone H3 phosphorylated at Threonine 3. No cross reactivity with other phosphorylated histones

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho-peptide corresponding to Phospho-Histone H3 (Thr3)

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3T3ph (phospho Thr3) antibody [RM159] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60897

GTX60897 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60897 Histone H3T3ph (phospho Thr3) antibody [RM159].
Red : Primary antibody
Green : Actin

Anti-Histone H3T3ph (phospho Thr3) antibody [RM159] used in Western Blot (WB). GTX60897

GTX60897 WB Image

Orthogonal

WB analysis of acid extracts of HeLa cells non-treated (1) or treated (2) with Nocodazole, and recombinant histone H3.3 (3) using GTX60897 Histone H3T3ph (phospho Thr3) antibody [RM159].
Dilution : 0.1μg/ml

Anti-Histone H3T3ph (phospho Thr3) antibody [RM159] used in IHC (Paraffin sections) (IHC-P). GTX60897

GTX60897 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX60897 Histone H3T3ph (phospho Thr3) antibody [RM159].
Dilution : 1μg/ml

Anti-Histone H3T3ph (phospho Thr3) antibody [RM159] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60897

GTX60897 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60897 Histone H3T3ph (phospho Thr3) antibody [RM159].
Red : Primary antibody
Green : Actin

REFERENCE

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REVIEW

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Package List Price ($)
$ 449