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Histone H3T3ph (phospho Thr3) antibody

Orthogonal
Anti-Histone H3T3ph (phospho Thr3) antibody used in Western Blot (WB). GTX130458
Anti-Histone H3T3ph (phospho Thr3) antibody used in Western Blot (WB). GTX130458
Anti-Histone H3T3ph (phospho Thr3) antibody used in Western Blot (WB). GTX130458

Cat. No. GTX130458

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
Not tested in other applications.

Positive Control

HeLa (0.1uM Calyculin treatment for 30 minutes+ phosphatase inhibitor) , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr3 of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886278

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3T3ph (phospho Thr3) antibody used in Western Blot (WB). GTX130458

GTX130458 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3T3ph (phospho Thr3) antibody (GTX130458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Histone H3T3ph (phospho Thr3) antibody used in Western Blot (WB). GTX130458

GTX130458 WB Image

Orthogonal

Histone H3 (phospho Thr3) antibody detects Histone H3 (phospho Thr3) protein by western blot analysis. Un-treated (-) and treated (+, 0.1uM Calyculin for 30 minutes + phosphatase inhibitor) HeLa whole cell extracts (2 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (GTX130458) at a dilution of 1:10000.

Anti-Histone H3T3ph (phospho Thr3) antibody used in Western Blot (WB). GTX130458

GTX130458 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3T3ph (phospho Thr3) antibody (GTX130458) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169