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Histone H3T3ph (phospho Thr3) antibody

Orthogonal
Anti-Histone H3T3ph (phospho Thr3) antibody used in Western Blot (WB). GTX79055
Anti-Histone H3T3ph (phospho Thr3) antibody used in IHC (Paraffin sections) (IHC-P). GTX79055

Cat. No. GTX79055

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Histone H3.1 around the phosphorylation site of Threonine 3 (A-R-Tp-K-Q).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11167331

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3T3ph (phospho Thr3) antibody used in Western Blot (WB). GTX79055

GTX79055 WB Image

Orthogonal

WB analysis of HUVEC cells treated with serum lysate using GTX79055 Histone H3T3ph (phospho Thr3) antibody.
Left : Primary antibody
Right : Primary antibody preincubated with immunogen peptide

Anti-Histone H3T3ph (phospho Thr3) antibody used in IHC (Paraffin sections) (IHC-P). GTX79055

GTX79055 IHC-P Image

IHC-P analysis of human malignant lymphoma tissue using GTX79055 Histone H3T3ph (phospho Thr3) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399