GeneTex
United States (US)

Histone H4 (Tri-Methyl Lys20) antibody [RM208]

Anti-Histone H4 (Tri-Methyl Lys20) antibody [RM208] used in Western Blot (WB). GTX33603
Anti-Histone H4 (Tri-Methyl Lys20) antibody [RM208] used in ELISA (ELISA). GTX33603

Cat No. GTX33603

Host Rabbit
Clonality Monoclonal
Clone Name RM208
Isotype IgG
Application WB, ELISA, Multiplexing
Reactivity Human
APPLICATION

Application Note

Western Blot: 1 ug/mL – 2 ug/mL, ELISA: 0.5 ug/mL - 1 ug/mL, Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Specificity/Sensitivity

This antibody reacts to Histone H4 trimethylated at Lysine 20 (K20me3). It may slightly cross react to dimethylated Lysine 20 (K20me2) at a higher concentration. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A trimethyl-peptide corresponding to trimethyl-Histone H4 (Lys20)

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Histone H4 (Tri-Methyl Lys20) antibody [RM208] used in Western Blot (WB). GTX33603

GTX33603 WB Image

Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM208 at 1 ug/mL,showed a band of Histone H4 trimethylated at Lysine20 in HeLa cells.

Anti-Histone H4 (Tri-Methyl Lys20) antibody [RM208] used in ELISA (ELISA). GTX33603

GTX33603 ELISA Image

RM208 specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) at higher concentrations. No crossreactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.

Package List Price ($)
$ 349