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Histone H4 (Tri-Methyl Lys20) antibody [RM208]

Anti-Histone H4 (Tri-Methyl Lys20) antibody [RM208] used in  (). GTX33603
Anti-Histone H4 (Tri-Methyl Lys20) antibody [RM208] used in Western Blot (WB). GTX33603

Cat. No. GTX33603

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM208

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
100 μg ($449)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL - 2 μg/mL
ELISA 0.5 μg/mL - 1 μg/mL
Not tested in other applications.

Product Note

This antibody reacts to Histone H4 trimethylated at Lysine 20 (K20me3). It may slightly cross react to dimethylated Lysine 20 (K20me2) at a higher concentration. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A trimethyl-peptide corresponding to trimethyl-Histone H4 (Lys20).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-Histone H4 (Tri-Methyl Lys20) antibody [RM208] used in  (). GTX33603

GTX33603 Image

The GTX33603 reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) at higher concentrations. No crossreactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.

Anti-Histone H4 (Tri-Methyl Lys20) antibody [RM208] used in Western Blot (WB). GTX33603

GTX33603 WB Image

WB analysis of extracts of HeLa cells (Lane 1) and recombinant Histone H4 protein (Lane 2) using GTX33603 Histone H4 (Tri-Methyl Lys20) antibody [RM208].
Dilution : 0.5μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 449