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Histone H4 antibody [HL1977]

Recombinant antibody
Anti-Histone H4 antibody [HL1977] used in IHC (Paraffin sections) (IHC-P). GTX637870
Anti-Histone H4 antibody [HL1977] used in IHC (Paraffin sections) (IHC-P). GTX637870
Anti-Histone H4 antibody [HL1977] used in Western Blot (WB). GTX637870
Anti-Histone H4 antibody [HL1977] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637870
Anti-Histone H4 antibody [HL1977] used in Western Blot (WB). GTX637870

Cat. No. GTX637870

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1977

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Histone H4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H4 antibody [HL1977] used in IHC (Paraffin sections) (IHC-P). GTX637870

GTX637870 IHC-P Image

Histone H4 antibody [HL1977] detects Histone H4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Histone H4 stained by Histone H4 antibody [HL1977] (GTX637870) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H4 antibody [HL1977] used in IHC (Paraffin sections) (IHC-P). GTX637870

GTX637870 IHC-P Image

Histone H4 antibody [HL1977] detects Histone H4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Histone H4 stained by Histone H4 antibody [HL1977] (GTX637870) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H4 antibody [HL1977] used in Western Blot (WB). GTX637870

GTX637870 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody [HL1977] (GTX637870) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H4 antibody [HL1977] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637870

GTX637870 ICC/IF Image

Histone H4 antibody [HL1977] detects Histone H4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 stained by Histone H4 antibody [HL1977] (GTX637870) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Histone H4 antibody [HL1977] used in Western Blot (WB). GTX637870

GTX637870 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody [HL1977] (GTX637870) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations