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Histone H4 antibody [RM212]

Anti-Histone H4 antibody [RM212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60913
Anti-Histone H4 antibody [RM212] used in Western Blot (WB). GTX60913
Anti-Histone H4 antibody [RM212] used in Western Blot (WB). GTX60913

Cat No. GTX60913

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM212

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1 μg/mL - 0.5 μg/mL
ICC/IF 1 μg/mL - 2 μg/mL
ELISA 0.2 μg/mL - 1 μg/mL
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Product Note

This antibody reacts to the Histone H4 protein, independent of post-translational modifications. No cross reactivity with other histone proteins.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.09% sodium azide, 50% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to the C-terminus of human Histone H4.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus,Chromosome

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H4 antibody [RM212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60913

GTX60913 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60913 Histone H4 antibody [RM212].
Red : Primary antibody
Green : Actin

Anti-Histone H4 antibody [RM212] used in Western Blot (WB). GTX60913

GTX60913 WB Image

WB analysis of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates using GTX60913 Histone H4 antibody [RM212].
Dilution : 0.2μg/ml

Anti-Histone H4 antibody [RM212] used in Western Blot (WB). GTX60913

GTX60913 WB Image

WB analysis of recombinant histone H2A, H2B,H3.1, H3.3 and H4 proteins using GTX60913 Histone H4 antibody [RM212].
Dilution : 0.2μg/ml

REFERENCE

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REVIEW

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