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Histone H4 antibody

Anti-Histone H4 antibody used in Dot blot (Dot). GTX129560
Anti-Histone H4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129560
Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560
Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560
Anti-Histone H4 antibody used in Western Blot (WB). GTX129560
Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560
Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560
Anti-Histone H4 antibody used in Western Blot (WB). GTX129560

Cat No. GTX129560

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, Dot
Reactivity Human, Mouse, Rat, Zebrafish
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:5000
IHC-P 1:100-1:1000
Dot 1:500
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , zebrafish brain , zebrafish eye , zebrafish whole

Predict Reactivity

Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

histone cluster 1, H4a Antibody , HIST1H4A Antibody , H4FA Antibody

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Research Area

DATA IMAGES
Anti-Histone H4 antibody used in Dot blot (Dot). GTX129560

GTX129560 Dot Image

Dot blot analysis of various of H4K20 peptides using Histone H4 antibody (GTX129560) at antibody dilution of 1:500.
Un : H4 protein peptide
Me : H4K20 mono-methyl peptide
2Me : H4K20 di-methyl peptide
3Me : H4K20 Tri-methyl peptide

Anti-Histone H4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129560

GTX129560 ICC/IF Image

Histone H4 antibody detects Histone H4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 protein stained by Histone H4 antibody (GTX129560) diluted at 1:5000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560

GTX129560 IHC-P Image

Histone H4 antibody detects Histone H4 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H4 antibody (GTX129560) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560

GTX129560 IHC-P Image

Histone H4 antibody detects Histone H4 protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H4 antibody (GTX129560) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H4 antibody used in Western Blot (WB). GTX129560

GTX129560 WB Image

Histone H4 antibody detects Histone H4 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody (GTX129560) diluted at a dilution of 1:5000.

Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560

GTX129560 IHC-P Image

Histone H4 antibody detects Histone H4 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Histone H4 antibody (GTX129560) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560

GTX129560 IHC-P Image

Histone H4 antibody detects Histone H4 protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
Histone H4 antibody (GTX129560) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H4 antibody used in Western Blot (WB). GTX129560

GTX129560 WB Image

Various tissue extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody (GTX129560) diluted at 1:1000.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169
Current in production