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Histone H4 antibody

Anti-Histone H4 antibody used in Western Blot (WB). GTX52365

Cat No. GTX52365

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μg ($319)

APPLICATION

Application Note

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Specificity/Sensitivity

HIST1H4A

PROPERTIES

Form

Liquid

Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Histone H4

Purification

Antigen affinity purification

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H4FA Antibody , histone cluster 1 , H4a Antibody , HIST1H4A Antibody

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES

Anti-Histone H4 antibody used in Western Blot (WB). GTX52365

GTX52365 WB Image

Western blot analysis of Histone H4 expression in TSA treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf