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Histone H4K20me1 (monomethyl Lys20) antibody

Orthogonal
Anti-Histone H4K20me1 (monomethyl Lys20) antibody used in Dot blot (Dot). GTX128959
Anti-Histone H4K20me1 (monomethyl Lys20) antibody used in Western Blot (WB). GTX128959
Anti-Histone H4K20me1 (monomethyl Lys20) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128959

Cat. No. GTX128959

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, Dot

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Histone H4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Dot 1:500
Not tested in other applications.

Positive Control

HeLa nuclear

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding mono-methyl Lys20 of human Histone H4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885847

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-Histone H4K20me1 (monomethyl Lys20) antibody used in Dot blot (Dot). GTX128959

GTX128959 Dot Image

Dot blot analysis of various of H4K20 methylation peptides using Histone H4K20me1 (monomethyl Lys20) antibody (GTX128959) at antibody dilution of 1:500.
Un : H4 protein peptide
Me : H4K20 mono-methyl peptide
2Me : H4K20 di-methyl peptide
3Me : H4K20 Tri-methyl peptide

Anti-Histone H4K20me1 (monomethyl Lys20) antibody used in Western Blot (WB). GTX128959

GTX128959 WB Image

Orthogonal

Histone H4 (mono methyl Lys20) antibody detects Histone H4 (mono methyl Lys20) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
15 % SDS-PAGE
Histone H4 (mono methyl Lys20) antibody (GTX128959) dilution: 1:1000

Anti-Histone H4K20me1 (monomethyl Lys20) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128959

GTX128959 ICC/IF Image

Histone H4 (mono methyl Lys20) antibody detects Histone H4 (mono methyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 (mono methyl Lys20) protein stained by Histone H4 (mono methyl Lys20) antibody (GTX128959) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Wang Y et al. PLoS One 2021; 16 (6) : e0253897 Comparative distributions of RSBN1 and methylated histone H4 Lysine 20 in the mouse spermatogenesis.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169