Histone H4K20me1 (monomethyl Lys20) antibody

Cat. No. GTX57170

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, Dot, ChIP assay
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all Histone H4 products

Datasheet
Document

View product citations for antibody GTX57170 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
ICC/IF	1:50 - 1:200
IHC-P	1:50 - 1:200
Dot	Assay dependent
ChIP assay	1:20 - 1:100

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human MonoMethyl-Histone H4-K20

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

GTX57170 ICC/IF Image

ICC/IF analysis of 293T cells using GTX57170 Histone H4K20me1 (monomethyl Lys20) antibody.
Blue : DAPI

GTX57170 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX57170 Histone H4K20me1 (monomethyl Lys20) antibody.

GTX57170 IHC-P Image

IHC-P analysis of rat testis tissue using GTX57170 Histone H4K20me1 (monomethyl Lys20) antibody.
Dilution : 1:200

GTX57170 WB Image

WB analysis of various sample lysates using GTX57170 Histone H4K20me1 (monomethyl Lys20) antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX57170 WB Image

WB analysis of C2C12 cell lysate using GTX57170 Histone H4K20me1 (monomethyl Lys20) antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX57170 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX57170 Histone H4K20me1 (monomethyl Lys20) antibody.
Dilution : 1:200

GTX57170 ChIP assay Image

ChIP analysis of HeLa cell lysate using GTX57170 Histone H4K20me1 (monomethyl Lys20) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

GTX57170 IHC-P Image

IHC-P analysis of human colon tissue using GTX57170 Histone H4K20me1 (monomethyl Lys20) antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

Histone H4K20me1 (monomethyl Lys20) antibody

Cat. No. GTX57170

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

DATA IMAGES

GTX57170 ICC/IF Image

GTX57170 Dot Image

GTX57170 IHC-P Image

GTX57170 WB Image

GTX57170 WB Image

GTX57170 IHC-P Image

GTX57170 ChIP assay Image

GTX57170 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.