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Histone H4K20me2 (dimethyl Lys20) antibody [GT1851]

Orthogonal
GTX630545_41582_20180810_DOT_18121411_995.jpg
GTX630545_41582_WB_Fraction_18121411_358.jpg
GTX630545_41582_ChIP_18121411_599.jpg
GTX630545_41582_IFA_18121411_381.jpg
GTX630545_41582_20141208_IP_18121411_524.jpg

Cat No. GTX630545

Host Mouse
Clonality Monoclonal
Clone Name GT1851
Isotype IgG1
Application WB, ICC/IF, IP, Dot, ChIP assay
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Dot 1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4K20me2 (dimethyl Lys20). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Histone H4 di-Methyl Lys20 Antibody, di-Methyl K20 Histone H4 Antibody, di Methyl K20 Histone H4 Antibody, H4FA Antibody, Histone H4 di-Methyl Antibody, Histone H4 di Methyl Antibody, di-Methyl Lys20 Histone H4 Antibody, di Methyl Histone H4 Lys20 Antibod

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Research Area

DATA IMAGES
GTX630545_41582_20180810_DOT_18121411_995.jpg

GTX630545 Dot Image

Dot blot analysis of various of H4K20 methylation peptides using Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (GTX630545) at antibody dilution of 1:500.
Un : H4 protein peptide
Me : H4K20 mono-methyl peptide
2Me : H4K20 di-methyl peptide
3Me : H4K20 Tri-methyl peptide

GTX630545_41582_WB_Fraction_18121411_358.jpg

GTX630545 WB Image

Orthogonal

Histone H4 (di methyl Lys20) antibody [GT1851] detects Histone H4 (di methyl Lys20) protein by western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg HeLa nuclear lysate/extract
Histone H4 (di methyl Lys20) antibody [GT1851] (GTX630545) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX630545_41582_ChIP_18121411_599.jpg

GTX630545 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

GTX630545_41582_IFA_18121411_381.jpg

GTX630545 ICC/IF Image

Histone H4 (di-Methyl Lys20) antibody [GT1851] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT1851] (GTX630545) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX630545_41582_20141208_IP_18121411_524.jpg

GTX630545 IP Image

Immunoprecipitation of HIST1H4A protein from HeLa whole cell extracts using 5 μg of Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (GTX630545).
Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (GTX630545).
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

REFERENCE
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169