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Histone H4K20me2 (dimethyl Lys20) antibody [GT1851]

Orthogonal
Anti-Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] used in Dot blot (Dot). GTX630545
Anti-Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] used in ChIP assay (ChIP assay). GTX630545
Anti-Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] used in Western Blot (WB). GTX630545
Anti-Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] used in Immunoprecipitation (IP). GTX630545
Anti-Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630545

Cat. No. GTX630545

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1851

Isotype

 IgG1

Application

 WB, ICC/IF, IP, Dot, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Histone H4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Dot 1:500
ChIP assay Assay dependent
Not tested in other applications.

Positive Control

HeLa , HeLa nuclear

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding di-methyl Lys20 of human Histone H4. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888218

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] used in Dot blot (Dot). GTX630545

GTX630545 Dot Image

Dot blot analysis of various of H4K20 methylation peptides using Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (GTX630545) at antibody dilution of 1:500.
Un : H4 protein peptide
Me : H4K20 mono-methyl peptide
2Me : H4K20 di-methyl peptide
3Me : H4K20 Tri-methyl peptide

Anti-Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] used in ChIP assay (ChIP assay). GTX630545

GTX630545 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

Anti-Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] used in Western Blot (WB). GTX630545

GTX630545 WB Image

Orthogonal

Histone H4 (di methyl Lys20) antibody [GT1851] detects Histone H4 (di methyl Lys20) protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
Histone H4 (di methyl Lys20) antibody [GT1851] (GTX630545) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] used in Immunoprecipitation (IP). GTX630545

GTX630545 IP Image

Immunoprecipitation of HIST1H4A protein from HeLa whole cell extracts using 5 μg of Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (GTX630545).
Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (GTX630545).
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630545

GTX630545 ICC/IF Image

Histone H4 (di-Methyl Lys20) antibody [GT1851] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT1851] (GTX630545) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Wang Y et al. PLoS One 2021; 16 (6) : e0253897 Comparative distributions of RSBN1 and methylated histone H4 Lysine 20 in the mouse spermatogenesis.
Application : WB
Reactivity : Mouse
Ye S et al. Cancer Res 2019; SET domain-containing protein 4 epigenetically controls breast cancer stem cell quiescence.
Application : WB
Reactivity : Human
Paquin KL et al. Mol Cell Biol 2019; (Epub) FANCD2 binding to H4K20me2 via a methyl-binding domain is essential for efficient DNA crosslink repair.
Application : WB
Reactivity : Human
Dai L et al. Mol Cell Biol 2016; (Epub) SETD4 regulates cell quiescence and catalyzes the trimethylation of H4K20 during diapause formation of Artemia.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations