GeneTex
United States (US)

Histone H4K20me3 (trimethyl Lys20) antibody

GTX128960_41416_20180810_DOT_18121410_630.jpg
GTX128960_41416_IFA_18121410_123.jpg
GTX128960_41416_IHC_M_2_18121410_346.jpg
GTX128960_41416_IHC_M_3_18121410_182.jpg
GTX128960_41416_WB_Fraction_18121410_441.jpg
GTX128960_41416_IHC_R_18121410_702.jpg
GTX128960_41416_IHC_M_18121410_471.jpg

Cat No. GTX128960

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, Dot
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Dot 1:500
Not tested in other applications.

Positive Control

HeLa , HeLa nuclear

Predict Reactivity

Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4K20me3 (trimethyl Lys20). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H4FA Antibody, histone cluster 1, H4a Antibody, HIST1H4A Antibody

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Research Area

DATA IMAGES
GTX128960_41416_20180810_DOT_18121410_630.jpg

GTX128960 Dot Image

Dot blot analysis of various of H4K20 methylation peptides using Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) at antibody dilution of 1:500.
Un : H4 protein peptide
Me : H4K20 mono-methyl peptide
2Me : H4K20 di-methyl peptide
3Me : H4K20 Tri-methyl peptide

GTX128960_41416_IFA_18121410_123.jpg

GTX128960 ICC/IF Image

Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 (tri methyl Lys20) protein stained by Histone H4 (tri methyl Lys20) antibody (GTX128960) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX128960_41416_IHC_M_2_18121410_346.jpg

GTX128960 IHC-P Image

Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128960_41416_IHC_M_3_18121410_182.jpg

GTX128960 IHC-P Image

Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128960_41416_WB_Fraction_18121410_441.jpg

GTX128960 WB Image

Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein by Western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg HeLa nuclear lysate/extract
15 % SDS-PAGE
Histone H4 (tri methyl Lys20) antibody (GTX128960) dilution: 1:2000

GTX128960_41416_IHC_R_18121410_702.jpg

GTX128960 IHC-P Image

Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128960_41416_IHC_M_18121410_471.jpg

GTX128960 IHC-P Image

Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REVIEW

Histone H4K20me3 (trimethyl Lys20) antibody Cat No. GTX128960

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 29-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Blocking : 5%milk in TBST
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169