Histone H4K20me3 (trimethyl Lys20) antibody

Name: Histone H4K20me3 (trimethyl Lys20) antibody
Brand: GeneTex
SKU: GTX128960
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Histone H4K20me3 (trimethyl Lys20) antibody used in Dot blot (Dot). GTX128960

Anti-Histone H4K20me3 (trimethyl Lys20) antibody used in IHC (Paraffin sections) (IHC-P). GTX128960

Anti-Histone H4K20me3 (trimethyl Lys20) antibody used in Western Blot (WB). GTX128960

Anti-Histone H4K20me3 (trimethyl Lys20) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128960

Cat. No. GTX128960

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, Dot
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Histone H4 products

Datasheet
Document

View product citations for antibody GTX128960 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
Dot	1:500

Not tested in other applications.

Positive Control

HeLa nuclear

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys20 of human Histone H4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885848

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

GTX128960 Dot Image

Dot blot analysis of various of H4K20 methylation peptides using Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) at antibody dilution of 1:500.
Un : H4 protein peptide
Me : H4K20 mono-methyl peptide
2Me : H4K20 di-methyl peptide
3Me : H4K20 Tri-methyl peptide

GTX128960 IHC-P Image

Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128960 WB Image

Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
15 % SDS-PAGE
Histone H4 (tri methyl Lys20) antibody (GTX128960) dilution: 1:2000

GTX128960 IHC-P Image

Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128960 IHC-P Image

Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128960 IHC-P Image

GTX128960 ICC/IF Image

Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 (tri methyl Lys20) protein stained by Histone H4 (tri methyl Lys20) antibody (GTX128960) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

There are currently no references for Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960). Be the first to share your publications with this product.

Submit a Reference

REVIEW