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Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody

Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653
Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653
Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in IHC (Paraffin sections) (IHC-P). GTX122653
Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653
Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Dot blot (Dot). GTX122653
Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122653

Cat No. GTX122653

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, Dot
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Dot Assay dependent
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

HeLa , NIH-3T3 , BCL-1 , PC-12 , *TGW ,

Predict Reactivity

Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

histone cluster 1 antibody, acetyl Histone H4 antibody, H4/a antibody, Histone4 acetyl antibody, HIST1H4B antibody, HIST2H4B antibody, member A antibody, HIST1H4E antibody, histone H4 antibody, HIST4H4 antibody, HIST1H4H antibody, HIST1H4K antibody, H4a antibody, HIST1H4A antibody, HIST1H4I antibody, HIST1H4F antibody, H4FA antibody, histone 1 antibody, HIST1H4C antibody, H4 histone family antibody, H4ac (pan acetyl) antibody, Acetyl Histone4 antibody, HIST1H4D antibody, HIST1H4L antibody, HIST1H4J antibody, HIST2H4A antibody

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Research Area

DATA IMAGES
Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653

GTX122653 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: BCL-1
15% SDS PAGE
GTX122653 diluted at 1:10000

Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653

GTX122653 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
15% SDS PAGE
GTX122653 diluted at 1:10000

Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in IHC (Paraffin sections) (IHC-P). GTX122653

GTX122653 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16)(GTX122653) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Western Blot (WB). GTX122653

GTX122653 WB Image

Sample (30 ug of whole cell lysate)
A: PC-12
15% SDS PAGE
GTX122653 diluted at 1:10000

Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Dot blot (Dot). GTX122653

GTX122653 Dot Image

Dotblot analysis of anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody with peptide samples.
Peptide samples (0.1 μg) were spotted onto positively charged nylon membrane and blotted with Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody (GTX122653) at different dilution as indicated.
A: Peptide samples of Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16)
B: Peptide samples of Histone H4

Anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122653

GTX122653 ICC/IF Image

Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody detects Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein stained by Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody (GTX122653) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

REFERENCE
REVIEW

Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody Cat No. GTX122653

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 10-Mar-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : TGW cells
Amount used : 30μg
Blocking : 5% Skim milk in TBST(0.1% tween-20), 25°C, 2Hr
Primary Antibody : 1:10000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169