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Histone H4K5ac (Acetyl Lys5) antibody [RM199]

Anti-Histone H4K5ac (Acetyl Lys5) antibody [RM199] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60905
Anti-Histone H4K5ac (Acetyl Lys5) antibody [RM199] used in  (). GTX60905
Anti-Histone H4K5ac (Acetyl Lys5) antibody [RM199] used in Western Blot (WB). GTX60905

Cat. No. GTX60905

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM199

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μg ($449)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2 μg/mL - 1 μg/mL
ICC/IF 0.5 μg/mL - 2 μg/mL
ELISA 0.2 μg/mL - 1 μg/mL
Not tested in other applications.

Product Note

This antibody reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with other acetylated Lysines in Histone H4.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys5).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-Histone H4K5ac (Acetyl Lys5) antibody [RM199] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60905

GTX60905 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60905 Histone H4K5ac (Acetyl Lys5) antibody [RM199].
Red : Primary antibody
Green : Actin

Anti-Histone H4K5ac (Acetyl Lys5) antibody [RM199] used in  (). GTX60905

GTX60905 Image

The GTX60905 reacts to Histone H4 acetylatedat Lysine 5 (K5ac). No cross reactivity with unmodifiedLysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac),Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.

Anti-Histone H4K5ac (Acetyl Lys5) antibody [RM199] used in Western Blot (WB). GTX60905

GTX60905 WB Image

WB analysis of extracts of HeLa cells (Lane 1) and recombinant Histone H4 protein (Lane 2) using GTX60905 Histone H4K5ac (Acetyl Lys5) antibody [RM199].
Dilution : 0.2μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 449