APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from hmuan Histone H4 (acetyl K5)
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
histone cluster 4 H4 , H4/p
Cellular Localization
Nucleus
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted throμgh the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Aμg 2015]
Database
Research Area
DATA IMAGES
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775](/templates/default/js/lightbox/images/loading.gif "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775")
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GTX00775 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4K5ac (acetyl Lys5) antibody [GT1138] (GTX00775) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX00775 WB Image
WB analysis of HeLa and C6 whole cell lysates using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Dilution : 1:1000
Loading : 25μg
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GTX00775 ICC/IF Image
ICC/IF analysis of C6 cells treated with/without TSA (1 μM at 37ºC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Orange: Primary antibody
Blue: DAPI
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GTX00775 ICC/IF Image
ICC/IF analysis of U2OS cells treated with/without TSA (1 μM at 37ºC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Orange: Primary antibody
Blue: DAPI
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GTX00775 IHC-P Image
IHC-P analysis of hmuan appendix tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Dlution : 1:100
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GTX00775 ICC/IF Image
ICC/IF analysis of NIH3T3 cells treated with/without TSA (1 μM at 37ºC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Orange: Primary antibody
Blue: DAPI
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GTX00775 IHC-P Image
IHC-P analysis of rat brain tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Dlution : 1:100
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GTX00775 IHC-P Image
IHC-P analysis of mouse kidney tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Dlution : 1:100
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REFERENCE
There are currently no references for Histone H4K5ac (acetyl Lys5) antibody [GT1138] (GTX00775). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Histone H4K5ac (acetyl Lys5) antibody [GT1138] (GTX00775). Be the first to share your experience with this product.
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775](/upload/website/prouct_img/small/GTX00775/GTX00775_40000000002_20200306_WB_treatment_Trichostatin_A_20031101_784.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775](/upload/website/prouct_img/small/GTX00775/GTX00775_20200327_WB_1_20040622_525.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775](/upload/website/prouct_img/small/GTX00775/GTX00775_20191101_AP_002_27_20040622_184.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775](/upload/website/prouct_img/small/GTX00775/GTX00775_20191101_AP_002_29_20040622_800.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775](/upload/website/prouct_img/small/GTX00775/GTX00775_20191101_AP_003_25_20040622_788.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775](/upload/website/prouct_img/small/GTX00775/GTX00775_20191101_AP_002_28_20040622_168.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775](/upload/website/prouct_img/small/GTX00775/GTX00775_20191101_AP_003_24_20040622_453.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775](/upload/website/prouct_img/small/GTX00775/GTX00775_20191101_AP_003_26_20040622_396.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775](/upload/website/prouct_img/normal/GTX00775/GTX00775_40000000002_20200306_WB_treatment_Trichostatin_A_20031101_784.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775](/upload/website/prouct_img/normal/GTX00775/GTX00775_20200327_WB_1_20040622_525.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775](/upload/website/prouct_img/normal/GTX00775/GTX00775_20191101_AP_002_27_20040622_184.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775](/upload/website/prouct_img/normal/GTX00775/GTX00775_20191101_AP_002_29_20040622_800.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775](/upload/website/prouct_img/normal/GTX00775/GTX00775_20191101_AP_003_25_20040622_788.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775](/upload/website/prouct_img/normal/GTX00775/GTX00775_20191101_AP_002_28_20040622_168.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775](/upload/website/prouct_img/normal/GTX00775/GTX00775_20191101_AP_003_24_20040622_453.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775")
![Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775](/upload/website/prouct_img/normal/GTX00775/GTX00775_20191101_AP_003_26_20040622_396.jpg "Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775")