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Histone H4K5ac (acetyl Lys5) antibody [GT1138]

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775

Cat No. GTX00775

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1138

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.3) with 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifμge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan Histone H4 (acetyl K5)

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone cluster 4 H4 , H4/p

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted throμgh the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Aμg 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775

GTX00775 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4K5ac (acetyl Lys5) antibody [GT1138] (GTX00775) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775

GTX00775 ICC/IF Image

ICC/IF analysis of C6 cells treated with/without TSA (1 μM at 37ºC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Orange: Primary antibody
Blue: DAPI

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775

GTX00775 ICC/IF Image

ICC/IF analysis of U2OS cells treated with/without TSA (1 μM at 37ºC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Orange: Primary antibody
Blue: DAPI

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775

GTX00775 IHC-P Image

IHC-P analysis of hmuan appendix tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Dlution : 1:100

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775

GTX00775 ICC/IF Image

ICC/IF analysis of NIH3T3 cells treated with/without TSA (1 μM at 37ºC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Orange: Primary antibody
Blue: DAPI

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775

GTX00775 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Dlution : 1:100

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775

GTX00775 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Dlution : 1:100

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775

GTX00775 WB Image

WB analysis of HeLa and C6 whole cell lysates using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Dilution : 1:1000
Loading : 25μg

REFERENCE

There are currently no references for Histone H4K5ac (acetyl Lys5) antibody [GT1138] (GTX00775). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Histone H4K5ac (acetyl Lys5) antibody [GT1138] (GTX00775). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369