*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Histone H4 (Acetyl-Lys5) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at lysine5.
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys5.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acet
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
H4F2, HIST, HIST1H4A, Histone H4, Histone H4 (acetyl K5), Histone H4 (acetyl Lys5), Histone H4 acetyl K5, Histone H4 acetyl Lys5, Acetyl Histone H4 (K5), Acetyl Histone H4 (Lys5)
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
GTX88005 WB Image
Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Histone H4 (Acetyl-Lys5) Antibody. The lane on the right is treated with the synthesized peptide.
GTX88005 IHC-P Image
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide.
GTX88005 ICC/IF Image
Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide.