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Histone H4K5ac (acetyl Lys5) antibody

Anti-Histone H4K5ac (acetyl Lys5) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88005
Anti-Histone H4K5ac (acetyl Lys5) antibody used in IHC (Paraffin sections) (IHC-P). GTX88005
Anti-Histone H4K5ac (acetyl Lys5) antibody used in Western Blot (WB). GTX88005

Cat. No. GTX88005

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Monkey
Package
100 μg ($399)
See all Histone H4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H4K5ac (1-50).

Purification

Purified by antigen-affinity chromatography. Non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

DATA IMAGES

Anti-Histone H4K5ac (acetyl Lys5) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88005

GTX88005 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX88005 Histone H4K5ac (acetyl Lys5) antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Histone H4K5ac (acetyl Lys5) antibody used in IHC (Paraffin sections) (IHC-P). GTX88005

GTX88005 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX88005 Histone H4K5ac (acetyl Lys5) antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Histone H4K5ac (acetyl Lys5) antibody used in Western Blot (WB). GTX88005

GTX88005 WB Image

WB analysis of COS7 cells treated with TSA 400nM (24h) lysate using GTX88005 Histone H4K5ac (acetyl Lys5) antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399