GeneTex
United States (US)

Histone H4K5ac (acetyl Lys5) antibody

Histone-H4-acetyl-Lys5-antibody-GTX88005-WB-1_18121411_919.jpg
Histone-H4-acetyl-Lys5-antibody-GTX88005-IHC-1_18121411_713.jpg
Histone-H4-acetyl-Lys5-antibody-GTX88005-ICC_IF-1_18121411_246.jpg

Cat No. GTX88005

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Specificity/Sensitivity

Histone H4 (Acetyl-Lys5) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at lysine5.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys5.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acet

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H4F2, HIST, HIST1H4A, Histone H4, Histone H4 (acetyl K5), Histone H4 (acetyl Lys5), Histone H4 acetyl K5, Histone H4 acetyl Lys5, Acetyl Histone H4 (K5), Acetyl Histone H4 (Lys5)

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Research Area

DATA IMAGES
Histone-H4-acetyl-Lys5-antibody-GTX88005-WB-1_18121411_919.jpg

GTX88005 WB Image

Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Histone H4 (Acetyl-Lys5) Antibody. The lane on the right is treated with the synthesized peptide.

Histone-H4-acetyl-Lys5-antibody-GTX88005-IHC-1_18121411_713.jpg

GTX88005 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide.

Histone-H4-acetyl-Lys5-antibody-GTX88005-ICC_IF-1_18121411_246.jpg

GTX88005 ICC/IF Image

Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide.