Histone H4K8ac (acetyl Lys8) antibody - ChIP grade

ChIP analysis of sheared chromatin from 10⁴ HeLa cells using GTX60829 Histone H4K8ac (acetyl Lys8) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the c-fos promoter and for the Sat2 satellite repeat region. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

ELISA analysis of peptide containing the histone modification of interest using GTX60829 Histone H4K8ac (acetyl Lys8) antibody - ChIP grade.

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H4K8 using GTX60829 Histone H4K8ac (acetyl Lys8) antibody - ChIP grade.
Dilution : 1:20,000

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60829 Histone H4K8ac (acetyl Lys8) antibody - ChIP grade.
Dilution : 1:200

ICC/IF analysis of 4% paraformaldehyde fixed NIH3T3 cells using GTX60829 Histone H4K8ac (acetyl Lys8) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500