Histone H4K8ac (acetyl Lys8) antibody - ChIP grade

Cat. No. GTX60829

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, Dot, ELISA, ChIP assay
Reactivity	Human, Mouse
Package	50 μg ($429)

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Datasheet

View product citations for antibody GTX60829 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:200
ICC/IF	1:500
Dot	1:20,000
ELISA	1:100
ChIP assay	1-5 μg

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region of histone H4 containing the acethylated lysine 8 (H4K8ac), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

GTX60829 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed NIH3T3 cells using GTX60829 Histone H4K8ac (acetyl Lys8) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

GTX60829 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60829 Histone H4K8ac (acetyl Lys8) antibody - ChIP grade.
Dilution : 1:200

GTX60829 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H4K8 using GTX60829 Histone H4K8ac (acetyl Lys8) antibody - ChIP grade.
Dilution : 1:20,000

GTX60829 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60829 Histone H4K8ac (acetyl Lys8) antibody - ChIP grade.

GTX60829 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁴ HeLa cells using GTX60829 Histone H4K8ac (acetyl Lys8) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the c-fos promoter and for the Sat2 satellite repeat region. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

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Package

List Price ($)

50 μg

$ 429

Qty

Histone H4K8ac (acetyl Lys8) antibody - ChIP grade

Cat. No. GTX60829

Host

Clonality

Isotype

Application

Reactivity