GeneTex

  • Country / Location Selection

United States (US)

Histone H4K8ac (acetyl Lys8) antibody [GT478]

Orthogonal
Anti-Histone H4K8ac (acetyl Lys8) antibody [GT478] used in IHC (Paraffin sections) (IHC-P). GTX633420
Anti-Histone H4K8ac (acetyl Lys8) antibody [GT478] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633420
Anti-Histone H4K8ac (acetyl Lys8) antibody [GT478] used in Western Blot (WB). GTX633420
Anti-Histone H4K8ac (acetyl Lys8) antibody [GT478] used in IHC (Paraffin sections) (IHC-P). GTX633420
Anti-Histone H4K8ac (acetyl Lys8) antibody [GT478] used in IHC (Paraffin sections) (IHC-P). GTX633420

Cat. No. GTX633420

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT478

Isotype

 IgG3

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Histone H4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

HeLa , HeLa (0.4μM Trichostatin A for 18 hr)

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys8 of human Histone H4. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888343

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-Histone H4K8ac (acetyl Lys8) antibody [GT478] used in IHC (Paraffin sections) (IHC-P). GTX633420

GTX633420 IHC-P Image

Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H4K8ac (acetyl Lys8) antibody [GT478] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633420

GTX633420 ICC/IF Image

Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4K8ac (acetyl Lys8) protein stained by Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420) diluted at 1:400.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102078) diluted at 1:300.
Scale bar = 10 μm.

Anti-Histone H4K8ac (acetyl Lys8) antibody [GT478] used in Western Blot (WB). GTX633420

GTX633420 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4K8ac (acetyl Lys8) antibody (GTX633420) diluted at 1:1000.

Anti-Histone H4K8ac (acetyl Lys8) antibody [GT478] used in IHC (Paraffin sections) (IHC-P). GTX633420

GTX633420 IHC-P Image

Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at nucleus in human ovary by immunohistochemical analysis.
Sample: Paraffin-embedded human ovary.
Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H4K8ac (acetyl Lys8) antibody [GT478] used in IHC (Paraffin sections) (IHC-P). GTX633420

GTX633420 IHC-P Image

Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Samata M et al. Cell 2020; 182 (1) : 127-144.e23 Intergenerationally Maintained Histone H4 Lysine 16 Acetylation Is Instructive for Future Gene Activation.
Application : WB
Submit a Reference

REVIEW

There are currently no reviews for Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations