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Histone H4R3me1 (Monomethyl Arg3) antibody [RM195]

Anti-Histone H4R3me1 (Monomethyl Arg3) antibody [RM195] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60904
Anti-Histone H4R3me1 (Monomethyl Arg3) antibody [RM195] used in Western Blot (WB). GTX60904
Anti-Histone H4R3me1 (Monomethyl Arg3) antibody [RM195] used in  (). GTX60904

Cat No. GTX60904

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM195

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL - 2 μg/mL
ICC/IF 1 μg/mL - 2 μg/mL
ELISA 0.2 μg/mL - 1 μg/mL
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Product Note

This antibody reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.09% sodium azide, 50% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A monomethyl -peptide corresponding to the Monomethyl -Histone H4 (Arg3).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus,Chromosome

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H4R3me1 (Monomethyl Arg3) antibody [RM195] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60904

GTX60904 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60904 Histone H4R3me1 (Monomethyl Arg3) antibody [RM195].
Red : Primary antibody
Green : Actin

Anti-Histone H4R3me1 (Monomethyl Arg3) antibody [RM195] used in Western Blot (WB). GTX60904

GTX60904 WB Image

WB analysis of extracts of HeLa cells (Lane 1) and recombinant Histone H4 protein (Lane 2) using GTX60904 Histone H4R3me1 (Monomethyl Arg3) antibody [RM195].
Dilution : 0.5μg/ml

Anti-Histone H4R3me1 (Monomethyl Arg3) antibody [RM195] used in  (). GTX60904

GTX60904 Image

The GTX60904 reacts to Histone H4 mono-methylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylatedArg 3 (R3me2a) or symmetric dimethylated Arg 3(R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.

REFERENCE

There are currently no references for Histone H4R3me1 (Monomethyl Arg3) antibody [RM195] (GTX60904). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Histone H4R3me1 (Monomethyl Arg3) antibody [RM195] (GTX60904). Be the first to share your experience with this product.