Histone H4R3me1 (Monomethyl Arg3) antibody [RM195]

Cat. No. GTX60904

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM195
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human
Package	100 μg ($449)

See all Histone H4 products

Datasheet

View product citations for antibody GTX60904 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.5 μg/mL - 2 μg/mL
ICC/IF	1 μg/mL - 2 μg/mL
ELISA	0.2 μg/mL - 1 μg/mL

Not tested in other applications.

Product Note

This antibody reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A monomethyl -peptide corresponding to the Monomethyl -Histone H4 (Arg3).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

GTX60904 Image

The GTX60904 reacts to Histone H4 mono-methylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylatedArg 3 (R3me2a) or symmetric dimethylated Arg 3(R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.

GTX60904 WB Image

WB analysis of extracts of HeLa cells (Lane 1) and recombinant Histone H4 protein (Lane 2) using GTX60904 Histone H4R3me1 (Monomethyl Arg3) antibody [RM195].
Dilution : 0.5μg/ml

GTX60904 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60904 Histone H4R3me1 (Monomethyl Arg3) antibody [RM195].
Red : Primary antibody
Green : Actin

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 449

Qty

Histone H4R3me1 (Monomethyl Arg3) antibody [RM195]

Cat. No. GTX60904

Host

Clonality

Clone Name

Isotype

Application

Reactivity