ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60904 Histone H4R3me1 (Monomethyl Arg3) antibody [RM195].
Red : Primary antibody
Green : Actin

WB analysis of extracts of HeLa cells (Lane 1) and recombinant Histone H4 protein (Lane 2) using GTX60904 Histone H4R3me1 (Monomethyl Arg3) antibody [RM195].
Dilution : 0.5μg/ml

The GTX60904 reacts to Histone H4 mono-methylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylatedArg 3 (R3me2a) or symmetric dimethylated Arg 3(R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.