GeneTex
United States (US)

Histone H4R3me2 (Symmetric dimethyl Arg3) antibody

GTX33909_20180530_ICCIF_18121410_570.jpg
GTX33909_20180530_WB_18121410_666.jpg
GTX33909_20180530_Dot_18121410_307.jpg

Cat No. GTX33909

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, Dot
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Dot Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide of human Symmetric DiMethyl-Histone H4-R3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H4 Antibody , H4F2 Antibody , H4FN Antibody , H4/N Antibody , HIST2H4A Antibody , FO108 Antibody , HIST2H4 Antibody

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES
GTX33909_20180530_ICCIF_18121410_570.jpg

GTX33909 ICC/IF Image

ICC/IF analysis of 293T cell using GTX33909 Histone H4R3me2 (Symmetric dimethyl Arg3) antibody.
Blue : DAPI

GTX33909_20180530_WB_18121410_666.jpg

GTX33909 WB Image

WB analysis of various samples using GTX33909 Histone H4R3me2 (Symmetric dimethyl Arg3) antibody.
Loading : 25μg per lane

GTX33909_20180530_Dot_18121410_307.jpg

GTX33909 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX33909 Histone H4R3me2 (Symmetric dimethyl Arg3) antibody.

Package List Price ($)
$ 319