GeneTex
United States (US)

Histone H4R3me2 (di-methyl Arg3) antibody

GTX54116_20180530_IHC-P_3_18121410_390.jpg
GTX54116_20180530_IHC-P_1_18121410_223.jpg
GTX54116_20180530_ICCIF_18121410_136.jpg
GTX54116_20180530_Dot_18121410_936.jpg
GTX54116_20180530_IHC-P_18121410_792.jpg
GTX54116_20180530_IHC-P_2_18121410_643.jpg
GTX54116_20180530_WB_18121410_969.jpg

Cat No. GTX54116

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, Dot
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:200
ICC/IF 1:50 - 1:20
IHC-P 1:50 - 1:20
Dot Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HIST2H4 Antibody , HIST2H4A Antibody , FO108 Antibody , H4/N Antibody , H4FN Antibody , H4F2 Antibody , H4 Antibody

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES
GTX54116_20180530_IHC-P_3_18121410_390.jpg

GTX54116 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX54116 Histone H4R3me2 (di-methyl Arg3) antibody.
Dilution : 1:200

GTX54116_20180530_IHC-P_1_18121410_223.jpg

GTX54116 IHC-P Image

IHC-P analysis of human rectal cancer tissue using GTX54116 Histone H4R3me2 (di-methyl Arg3) antibody.
Dilution : 1:200

GTX54116_20180530_ICCIF_18121410_136.jpg

GTX54116 ICC/IF Image

ICC/IF analysis of 293T cell using GTX54116 Histone H4R3me2 (di-methyl Arg3) antibody.
Blue : DAPI

GTX54116_20180530_Dot_18121410_936.jpg

GTX54116 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX54116 Histone H4R3me2 (di-methyl Arg3) antibody.

GTX54116_20180530_IHC-P_18121410_792.jpg

GTX54116 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX54116 Histone H4R3me2 (di-methyl Arg3) antibody.
Dilution : 1:200

GTX54116_20180530_IHC-P_2_18121410_643.jpg

GTX54116 IHC-P Image

IHC-P analysis of rat brain tissue using GTX54116 Histone H4R3me2 (di-methyl Arg3) antibody.
Dilution : 1:200

GTX54116_20180530_WB_18121410_969.jpg

GTX54116 WB Image

WB analysis of various samples using GTX54116 Histone H4R3me2 (di-methyl Arg3) antibody.
Loading : 25μg per lane

Package List Price ($)
$ 319