Histone H4S1ph (phospho Ser1) antibody [RM194]

Cat. No. GTX60903

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM194
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human
Package	100 μg ($449)

See all Histone H4 products

Datasheet

View product citations for antibody GTX60903 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.5 μg/mL - 2 μg/mL
ICC/IF	1 μg/mL - 2 μg/mL
ELISA	0.2 μg/mL - 1 μg/mL

Not tested in other applications.

Product Note

This antibody reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighboring Arginine 3 modifications. No cross reactivity with Histone H2A phosphorylated at Serine 1.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho-peptide corresponding to the Phospho-Histone H4 (pSer1).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

GTX60903 WB Image

WB analysis of extracts of HeLa cells (Lane 1) and recombinant Histone H4 protein (Lane 2) using GTX60903 Histone H4S1ph (phospho Ser1) antibody [RM194].
Dilution : 1μg/ml

GTX60903 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60903 Histone H4S1ph (phospho Ser1) antibody [RM194].
Red : Primary antibody
Green : Actin

GTX60903 Image

The GTX60903 reacts to Histone H4 phosphorylatedat Serine 1 (H4S1p). The reactivity is not affected byneighboring Arginine 3 modifications (H4S1pR3me1and H4S1pR3me2). No cross reactivity with Histone H2Aphosphorylated at Serine 1 (H2AS1p).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 449

Qty

Histone H4S1ph (phospho Ser1) antibody [RM194]

Cat. No. GTX60903

Host

Clonality

Clone Name

Isotype

Application

Reactivity