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Homer1 antibody

Anti-Homer1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103278
Anti-Homer1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX103278
Anti-Homer1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103278
Anti-Homer1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103278
Anti-Homer1 antibody used in Western Blot (WB). GTX103278
Anti-Homer1 antibody used in Western Blot (WB). GTX103278
Anti-Homer1 antibody used in Immunoprecipitation (IP). GTX103278

Cat No. GTX103278

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Homer1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950505

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

homer scaffold protein 1 , HOMER , HOMER1A , HOMER1B , HOMER1C , SYN47 , Ves-1

Cellular Localization

Cytoplasm , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse

Background

This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Homer1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103278

GTX103278 ICC/IF Image

Homer1 antibody detects Homer1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Homer1 protein stained by Homer1 antibody (GTX103278) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Homer1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX103278

GTX103278 IHC-Fr Image

Homer antibody detects Homer protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Homer protein stained by Homer antibody (GTX103278) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Homer1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103278

GTX103278 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HOMER1(GTX103278) antibody at 1:100 dilution.

Anti-Homer1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103278

GTX103278 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Homer(GTX103278) antibody at 1:500 dilution.

Anti-Homer1 antibody used in Western Blot (WB). GTX103278

GTX103278 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GTX103278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Homer1 antibody used in Western Blot (WB). GTX103278

GTX103278 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GTX103278) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Homer1 antibody used in Immunoprecipitation (IP). GTX103278

GTX103278 IP Image

Immunoprecipitation of Homer protein from IMR32 whole cell extracts using 5 μg of Homer antibody (GTX103278).
Western blot analysis was performed using Homer antibody (GTX103278).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169