GeneTex
United States (US)

Homer1 antibody

GTX103278_39967_20170705_IFA_R_19011506_447.jpg
GTX103278_39967_20160921_IHC-Fr_19011506_684.jpg
GTX103278_41745_20170518_WB_M_R_19011506_610.jpg
GTX103278_IHC_19011506_810.jpg
GTX103278_39967_IFA_19011506_111.jpg
GTX103278_43250_20180622_WB_19021719_383.jpg
GTX103278_43425_20181221_WB_19030801_831.jpg
GTX103278_39967_20150618_IP_19011506_510.jpg

Cat No. GTX103278

Reference 3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
See all Homer1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Homer1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Homer Scaffold Protein 1,Homer,Homer1A,Homer1B,Homer1C,Syn47,Ves-1,Homer1

Cellular Localization

Cytoplasm , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse

Background

This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103278_39967_20170705_IFA_R_19011506_447.jpg

GTX103278 ICC/IF Image

Homer1 antibody detects Homer1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Homer1 protein stained by Homer1 antibody (GTX103278) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GTX103278_39967_20160921_IHC-Fr_19011506_684.jpg

GTX103278 IHC-Fr Image

Homer antibody detects Homer protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Homer protein stained by Homer antibody (GTX103278) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GTX103278_41745_20170518_WB_M_R_19011506_610.jpg

GTX103278 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GTX103278) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX103278_IHC_19011506_810.jpg

GTX103278 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HOMER1(GTX103278) antibody at 1:100 dilution.
GTX103278_39967_IFA_19011506_111.jpg

GTX103278 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Homer(GTX103278) antibody at 1:500 dilution.
GTX103278_43250_20180622_WB_19021719_383.jpg

GTX103278 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GTX103278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX103278_43425_20181221_WB_19030801_831.jpg

GTX103278 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GTX103278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX103278_39967_20150618_IP_19011506_510.jpg

GTX103278 IP Image

Immunoprecipitation of Homer protein from IMR32 whole cell extracts using 5 μg of Homer antibody (GTX103278).
Western blot analysis was performed using Homer antibody (GTX103278).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
REFERENCE

Application Reference

Penney J et al. J. Neurosci. 2017; (Epub ahead of print) Loss of protein arginine methyltransferase 8 alters synapse composition and function, resulting in behavioral defects.
Application : WB
Reactivity : Mouse
Haider A et al. Alcohol 2015; 49 (6) : 533-42 Homer2 within the nucleus accumbens core bidirectionally regulates alcohol intake by both P and Wistar rats.
Application : WB
Reactivity : Rat
Cozzoli DK et al. Neuropsychopharmacology 2014; 39 (2) : 435-44 Binge alcohol drinking by mice requires intact group 1 metabotropic glutamate receptor signaling within the central nucleus of the amygdala.
Application : WB
Reactivity : Mouse
Submit a Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169