APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain
Predict Reactivity
Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.75 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Homer1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950505
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
homer scaffold protein 1 , HOMER , HOMER1A , HOMER1B , HOMER1C , SYN47 , Ves-1
Cellular Localization
Cytoplasm , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse
Background
This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX103278 ICC/IF Image
Homer1 antibody detects Homer1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Homer1 protein stained by Homer1 antibody (GTX103278) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX103278 IHC-Fr Image
Homer antibody detects Homer protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Homer protein stained by Homer antibody (GTX103278) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX103278 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HOMER1(GTX103278) antibody at 1:100 dilution.
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GTX103278 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using Homer(GTX103278) antibody at 1:500 dilution.
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GTX103278 IP Image
Immunoprecipitation of Homer protein from IMR32 whole cell extracts using 5 μg of Homer antibody (GTX103278).
Western blot analysis was performed using Homer antibody (GTX103278).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX103278 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GTX103278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103278 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GTX103278) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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