GeneTex
United States (US)

Homer3 antibody

GTX115242_40254_20170705_IFA_R_18121410_748.jpg
GTX115242_IHC_18121410_519.jpg
GTX115242_40254_IFA_18121410_789.jpg
GTX115242_WB_18121410_712.jpg

Cat No. GTX115242

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Homer3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Homer Scaffold Protein 3,Homer-3,Vesl3,Homer3

Cellular Localization

Cytoplasm , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse

Background

This gene encodes a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides. Alternative splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES
GTX115242_40254_20170705_IFA_R_18121410_748.jpg

GTX115242 ICC/IF Image

Homer3 antibody detects Homer3 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Homer3 protein stained by Homer3 antibody (GTX115242) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX115242_IHC_18121410_519.jpg

GTX115242 IHC-P Image

Homer3 antibody detects Homer3 protein at cytoplasm on human Papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded Papillary adenocarcinoma .
Homer3 antibody (GTX115242) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115242_40254_IFA_18121410_789.jpg

GTX115242 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Homer3(GTX115242) antibody at 1:500 dilution.

GTX115242_WB_18121410_712.jpg

GTX115242 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX115242 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169