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Hsp40 antibody [k1C7]

Anti-Hsp40 antibody [k1C7] used in Western Blot (WB). GTX50059

Cat No. GTX50059

Host

Mouse

Clonality

Monoclonal

Clone Name

k1C7

Isotype

IgG2b

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Hsp40(1-340aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dnaj Heat Shock Protein Family (Hsp40) Member B1 , Hspf1 , Hdj1 , Hsp40 , Rsph16B , Sis1 , Dnajb1

Cellular Localization

Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.

Background

Human Hsp40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. Hsp40 protein contains the J-domain which controls interaction with the ATPase domain of Dnak.

Database

Research Area

DATA IMAGES

Anti-Hsp40 antibody [k1C7] used in Western Blot (WB). GTX50059

GTX50059 WB Image

Cell lysates of Jurkat (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp40 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf