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Hsp40 antibody [k1C7]

Anti-Hsp40 antibody [k1C7] used in Western Blot (WB). GTX50059

Cat. No. GTX50059

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 k1C7

Isotype

 IgG2b

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Hsp40 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Hsp40(1-340aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member B1 , HSPF1 , Hdj1 , Hsp40 , RSPH16B , Sis1

Cellular Localization

Cytoplasm , Nucleus , Nucleolus

Background

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-Hsp40 antibody [k1C7] used in Western Blot (WB). GTX50059

GTX50059 WB Image

Cell lysates of Jurkat (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp40 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399