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Hsp40 antibody

KO/KD
Anti-Hsp40 antibody used in Western Blot (WB). GTX54013
Anti-Hsp40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54013
Anti-Hsp40 antibody used in Western Blot (WB). GTX54013
Anti-Hsp40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54013
Anti-Hsp40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54013

Cat. No. GTX54013

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Hsp40 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member B1 , HSPF1 , Hdj1 , Hsp40 , RSPH16B , Sis1

Cellular Localization

Cytoplasm,Nucleus,Nucleolus

Background

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-Hsp40 antibody used in Western Blot (WB). GTX54013

GTX54013 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX54013 Hsp40 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Hsp40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54013

GTX54013 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54013 Hsp40 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Hsp40 antibody used in Western Blot (WB). GTX54013

GTX54013 WB Image

WB analysis of various sample lysates using GTX54013 Hsp40 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Hsp40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54013

GTX54013 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54013 Hsp40 antibody.
Blue : DAPI

Anti-Hsp40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54013

GTX54013 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54013 Hsp40 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399